Originally, Ham's Nutrient Mixtures were developed for the clonal growth of several Chinese hamster ovary (CHO) clone cells, Hela cells and mouse L-cells. Both F-10 and F-12 are formulated for use with or without serum, depending on the type of cells being cultured. F-10 has demonstrated satisfactory growth of human diploid cells, white blood cells for chromosomal analysis and primary rat, rabbit and chicken tissue explants. Ham's F-12 is well suited for the growth of primary rat hepatocytes and prostate epithelial cells. It is also the medium of choice for toxicity assays using CHO cells. MP also offers Ham's F-12 supplemented with 25 mM HEPES for improved buffering in the optimum pH range of 7.2-7.4. F12K is a modification of Ham's F-12 and Coon's F-12 with increased amino acid and pyruvate concentrations. Additionally, the salts have been modifed (Konig
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Mammalian Cell Culture
SKU | 091242354 |
Base Catalog Number | 091242354 |
Concentration | 1X |
Format | Solution |
Formulation Details | Liquid |
Half Life | 6 months |
Hazard Statements | No |
Media Type | Ham's F12 |
Pack Size | 500 mL |
Typical Working Concentration | Liquid media for cell culture |
Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |