RNaid® SPIN KIT provides a simple and convenient way to isolate total RNA from agarose and polyacrylamide gels and from solutions. It Purify RNA fragments from 75 bases to 10 kb.
Application Notes
The RNaid® Spin Kit is designed to isolate and purify RNA (75 bases to 10 kb) from native and denaturing agarose and polyacrylamide gels. Removal of unincorporated nucleotides, buffer salts, and other reagents from enzyme reactions. The purified RNA is ready for reverse transcription and PCR, labeling and microarray analysis, and other common applications.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Isolate and purify RNA from native and denaturing agarose and polyacrylamide gels and from solutions
SKU | 111107200 |
Alternate Names | RNA Clean-Up Kit |
Application Notes | The RNaid® Spin Kit is designed to isolate and purify RNA (75 bases to 10 kb) from native and denaturing agarose and polyacrylamide gels. Removal of unincorporated nucleotides, buffer salts, and other reagents from enzyme reactions. The purified RNA is ready for reverse transcription and PCR, labeling and microarray analysis, and other common applications. |
Base Catalog Number | 1107200 |
Brand | RNaid™ |
Components | DEPC-Treated Water |RNA Binding Salt |RNA Wash Concentrate |RNAMATRIX® |10% Acetic Acid |SPIN Filters |Catch Tubes |
Format | RNAMATRIX™ slurry plus SPIN filter |
Pack Size | 200 Preps |
Personal Protective Equipment | Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice. |
Preparation Method | Before first use, add the 120 mL of 200 proof (100% ethanol) to the RNA Wash Concentrate and mix well. Do not use denatured alcohol because it can cause precipitation of salts. Label the container. Store tightly capped at 15°-30°C. |
Recommended Alternate | RNaid™ Kit |
Sample Source | Gels and Solutions |
Sample Type | Enzymatic Reactions, Solutions, Agarose Gels, Polyacrylamide Gels |
Sample Volume | Up to ~100 mg gel slice or up to ~100 µl DNA solution |
Unit Definition | One µL of RNAMATRIX™ will bind one µg of RNA. |
Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |