FBS, US Origin is collected from a U.S.D.A. regulated and monitored abattoir by technicians who work "on-site" where the fetal blood is aseptically collected via cardiac puncture. This raw material is transported to the production facility where it undergoes sterilization via filtration through multiple 0.1 micron filters. The care and diligence taken in handling the fetal serum minimizes cell lysis and other detrimental factors thereby reducing possible lysozyme and hemoglobin contamination. This translates into increased performance in the final product. It is superior for hybridoma cloning. It is supplied sterile, with no detectable mycoplasma or viral infection. Serum is an extremely complex mixture of many small and large biomolecules with different, physiologically balanced growth-promoting and growth inhibiting activities.
Application Notes
FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems. It is typically used with the classical media. These include many popular media such as Minimum Essential Medium (MEM), Dulbecco′s Modified Eagles Media (DME, DMEM), Iscove′s Modified Eagles Medium (IMDM), M199 and Roswell Park Memorial Institute medium, RPMI-1640. Some of the major functions of serum are to provide growth factors, hormones, attachment and spreading factors, binding proteins, lipids, and minerals. FDA qualified for IVD applications.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Mammalian Cell Culture | Stem Cell Culture
SKU | 0929167-CF |
Alternate Names | FBS |
Application Notes | FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems. It is typically used with the classical media. These include many popular media such as Minimum Essential Medium (MEM), Dulbecco′s Modified Eagles Media (DME, DMEM), Iscove′s Modified Eagles Medium (IMDM), M199 and Roswell Park Memorial Institute medium, RPMI-1640. Some of the major functions of serum are to provide growth factors, hormones, attachment and spreading factors, binding proteins, lipids, and minerals. FDA qualified for IVD applications. |
Base Catalog Number | 2916749 |
Concentration | Protein 3.1 - 4.4 g/dL |
Format | Liquid |
Formulation Details | Liquid | Glucose 116 mg/dL Sodium 140 mEq/L BUNN 13 mg/dL Chloride 100 mEq/L Creatinine 2.4 mg/dL Uric Acid 3.2 mg/dL Calcium 11.9 mg/dL Phosphorus 8 mg/dL Albumin 2.6 g/dL Total Bilirubin 0.3 mg/dL Triglyceride 32 mg/dL Alkaline phosphatase 168 U/L AST (SGOT) 35 U/L LDH 585 U/L Cholesterol <40 mg/dL Triglycerides 0.43 mmol/L IgG Content <60 mg/L GGT 7 U/L Electrophoretic Pattern Normal Alpha1 3 g/L Alpha 2 11 g/L Beta 4 g/L Gamma 1 g/L | Liquid |
pH | 6.7 - 7.8 |
Sterility | Sterile | Filtration through multiple .1 micron filters |
Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |