Betaine
Application Notes
Betaine is a reagent that is used in soldering, resin curing fluxes, and organic synthesis. Betaine has been studied in its role in protecting plants from environmental stress and in maintaining osmolarity in mammalian cells. Betaine has been reported to diminish the base pair composition dependence of DNA thermal melting transitions, with a concentration of 5.2 M betaine being the value at which AT and GC base pairs are equally stable and hence subsequently found application in the polymerase chain reaction (PCR). The usefulness of betaine in PCR has resulted from its ability to enhance DNA amplification by diminishing the formation of secondary structure in GC-rich DNA regions. A protocol for the production of long-chain cDNAs that uses betaine and trehalose has been published. The permanent positive charge on betaine makes it a target of study for mass spectrometry (MS) analysis by such methods as electrospray ionization (ESI) MS.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
| SKU | 02101003-CF |
| Alternate Names | Lycine; Oxyneurine; (Carboxymethyl)trimethylammonium inner salt; Glycine betaine; 1-carboxy-N,N,N-trimethylmethanammonium inner salt |
| Application Notes | Betaine is a reagent that is used in soldering, resin curing fluxes, and organic synthesis. Betaine has been studied in its role in protecting plants from environmental stress and in maintaining osmolarity in mammalian cells. Betaine has been reported to diminish the base pair composition dependence of DNA thermal melting transitions, with a concentration of 5.2 M betaine being the value at which AT and GC base pairs are equally stable and hence subsequently found application in the polymerase chain reaction (PCR). The usefulness of betaine in PCR has resulted from its ability to enhance DNA amplification by diminishing the formation of secondary structure in GC-rich DNA regions. A protocol for the production of long-chain cDNAs that uses betaine and trehalose has been published. The permanent positive charge on betaine makes it a target of study for mass spectrometry (MS) analysis by such methods as electrospray ionization (ESI) MS. |
| Base Catalog Number | 101003 |
| Beilstein Registry Number | 3537113 |
| Biochemical Physiological Actions | End-product of oxidative metabolism of choline, betaine is a general methyl donor, in particular in a minor pathway of methionine biosynthesis. It is used to treat homocystinuria, which is a defect in the major pathway of methionine biosynthesis. |
| CAS # | 107-43-7 |
| EC Number | 203-490-6 |
| Format | Powder |
| Grade | Anhydrous |
| Melting Point | Decomposes around 293 deg C |
| Molecular Formula | C5H11NO2 |
| Molecular Weight | 117.148 g/mol |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| pKa | 1.83 (Lit.) |
| RTECS Number | DS5900000 |
| Solubility | Solubility (g/100 g solvent): <a class="pubchem-internal-link CID-887" href="https://pubchem.ncbi.nlm.nih.gov/compound/methanol">methanol</a>, 55; <a class="pubchem-internal-link CID-702" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethanol">ethanol</a>, 8.7. Sparingly soluble in ether. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| Vapor Pressure | 1.36X10-8 mm Hg at 25 deg C (est) |
![MP COA [101003] lot ID: 1646J](https://www.mpbiochina.com/static/version1713424798/frontend/Mpbio/default/en_US/images/document.png "MP COA [101003] lot ID: 1646J")
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