Genomic DNA Isolation

Shopping Options

• FastPrep Models
  All FastPrep Adapters ConeFlex Legacy (allows all FP24 adapters on FP96) CoolPrep (24x2 mL) HiPrep (48x2 mL) QuickFlex (96x2 mL) QuickPrep (24x2 mL) 2 x 96 Well Adapter BigFlex (8x50 mL) BigPrep (2x50 mL) CoolBigPrep (2x50 mL)
• Capacity
  Bacterial Colony: 200-400 µL /1-3 colony Bacterial Pellet: 200-400 µL /Up to 109 bacteria (about OD600 = 3) cell-Free body fluids: 200-400 µL cell-free body fluids Liquid Transport media: 200-400µL liquid transport media Tissue: 200-400 µL /1-30 mg Urine: 200-400 µL /5-50 mL urine 400-1000 µL buffy coat* (contain no more than 5x 106 cells) 400-1000 µL whole blood (WBC count less than 6x 104 cells/µL) 100-400 µL buffy coat 100-400 µL whole blood (WBC count less than 2x 10000 cells/µL) 100-400µL leukocyte concentration (contain no more than 5 x 106 cells) 100-400 µL/ One to eight 10 µm- thick sections of tissue. 100-400 µL/ three to ten biopsies. Chewing Gum - 40 mg Cigarette butts - 1 cm2 paper from the end of the cigarette or filter Clotted/ dried blood - 20 µL Forensic Surface and Contact Swabs Hair root - 0.5–1 cm Human tissues - 40mg Saliva - 50µL Sperm Stains - 5-10 µL or 1 cm2 of the forensic sample Swab Samples 400 µL 100-400 µL / 1 swab or brush for Dried Swab samples 100-400 µL / 4 discs for dried blood Purify DNA from 100-400 µL / 10-40mg of tissue Purify Nucleic Acids from 100 to 400 µL of sample 600-800 µl Bacterial Colony: 100-200 µl /1-3 colony Bacterial Pellet: 100-200 µl /Up to 109 bacteria (about OD600 = 3) Swab Samples: 100-200 µl liquid transport media 500-700 µL
• Sample Type
  Blood Cell Culture Tissue Saliva Seeds Spores Bacteria (Gram +/-) Animal Tissues Feces Food Fungi Plants Roots Soil Wastewater Yeast Algae Mammalian Stool (i.e. humans, primates, birds, rodents, cattle, canine, feline, porcine, etc.) Buds Flowers Fruits Leaves Stems Environmental Water Sludge Cultured Cells Insects Bacteria Plant Animal Tissue Forensic samples Swabs Bacterial cells 400-1000 µL whole blood (WBC count less than 6x 104 cells/µL) 400-1000µL buffy coat* (contain no more than 5x 106 cells) 100-400 µL leukocyte concentration (contain no more than 5x 106 cells) 100-400 µL whole blood (WBC count less than 2x 104 cells/µL) 100-400µL buffy coat FFPE Tissues Fungal Tissues Plant (leaf, seeds and spores) Blood Stain Swab Human biological specimens such as serum, plasma and other cell-free fluids Bacterial Viral Lysate or other suspension Plant tissues including plant cells, lichen, rice, grains, leaves, stems, buds, fruit, and seeds; 50 – 150 mg input Water samples including fresh and sea water, pond and river water, wastewater, etc. Bodily Fluid Up to 500 mg of soil samples, including sand, alkaline soil, clay, sediment, and topsoil Up to 250 mg per prep of fecal/stool samples Swabs, sputum, body fluids, stool, and environmental samples (e.g. soil and water)
• Format
  Kit All necessary reagents Kit Component Geneclean® slurry plus SPIN filter SPIN filter and Catch Tube
• Product Line
  GENECLEAN® FastDNA-96™ FastDNA™ FastDNA™ GNOME® MPure-12™ SPINeasy MagBeads