Tris-glycine buffer, 1 box

Name: Tris-glycine buffer, 1 box
SKU: 04816200

Key features and details

Tris-Glycine buffer for protein gel electrophoresis and western blotting.

SKU: 04816200

CAS Number:

Not applicable

Packaging size:

1 box

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Product Description

Tris-Glycine Buffer

Application Notes

Tris-Glycine Buffer is used for Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Used for Western blotting and gel electrophoresis

Specifications

SKU	04816200
Application Notes	Tris-Glycine Buffer is used for Western blotting and gel electrophoresis. Tris-glycine buffer is used to make a Tris-glycine-methanol transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during the transfer and enhances the protein binding to nitrocellulose.
Base Catalog Number	816200
CAS #	Not applicable
Components	0.025 M Tris, 0.192 M Glycine
Format	Powder
Pack Size	1 box
Personal Protective Equipment	Eyeshields, full-face respirator, Gloves, multi-purpose combination respirator cartridge, respirator filter
Preparation Method	Empty contents of 1 packet into a 4-liter flask, add 800 mL of methanol and QS to volume with deionized water. Four liters of pH 8.3 buffer is now ready to use.
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.