Poly-L-Lysine Hydrobromide
Application Notes
Poly-L-Lysine is typically used as a coating substrate for culture dishes, slides, etc. It can be used in conjugation to methotrexate for increased drug transport. For Microencapsulation of islets. It is also use in simple reproducible procedure for chromosomal preparations from a variety of tissues. For immobilation of living cells in biocompatible semipermeable microcapsules. In the preparation of polycationic beads. Conjugation to albumin and horseradish peroxidase to enhance cellular uptake.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Coating substrate for culture dishes; slides | Cell adhesion
| SKU | 02152689-CF |
| Application Notes | Poly-L-Lysine is typically used as a coating substrate for culture dishes, slides, etc. It can be used in conjugation to methotrexate for increased drug transport. For Microencapsulation of islets. It is also use in simple reproducible procedure for chromosomal preparations from a variety of tissues. For immobilation of living cells in biocompatible semipermeable microcapsules. In the preparation of polycationic beads. Conjugation to albumin and horseradish peroxidase to enhance cellular uptake. |
| Base Catalog Number | 152689 |
| Biochemical Physiological Actions | Poly-L-Lysine hydrobromide is a synthetic amino acid that enhances cell adhesion to solid substrates. It has been proven useful as a vector for gene therapy. Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor. |
| CAS # | 25988-63-0 |
| Format | Powder |
| Molecular Weight | 402.54 g/mol |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| Typical Working Concentration | Stock Solution for coating of culture dishes/slides: Dissolve 10 mg poly-L-lysine in 1 mL sterile, deionized water. This creates a 1% solution. Effective coating solution concentration for culture dishes will be 0.3 to 0.5% and for histology slides at 0.1%. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [152689] lot ID: 2173J](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [152689] lot ID: 2173J")