Chloroform
Application Notes
Chloroform is use as a solvent for fats, oils, rubber, alkaloids, waxes, gutta-percha, resins; as cleansing agent; in fire extinguishers to lower the freezing temperature of carbon tetrachloride. Procedures have been described for the use of chloroform in lambda plaques storage, lambda cDNA storage, the removal of mineral oil from PCR reaction samples, oligonucleotide purification, a hydroxyl radical footprinting protocol, a transcriptional run-on assay protocol, and an overlay assay for beta-galactosidase activity. It has also been used with phenol in such procedures as DNA recovery from polyacrylamide gels, ethidium bromide removal from DNA preparations, lysis protocols for plasmid DNA isolation, RNase removal, and purification of yeast DNA. A protocol describes the use of chloroform in a high-performance thin-layer chromatography protocol for sphingomyelin analysis. Chloroform can also be used in chloroform/methanol mixtures for the isolation of cardiolipids from Geobacillus stearothermophilus and their subsequent MS analysis. The isolation of the bacteriocin amylovorin L471 from Lactobacillus amylovorus DCE 471 in culture broth has been reported, using chloroform/methanol extraction and precipitation in the procedure. Chloroform/2-butanol mixtures can be used for the extraction of steroid sulfates for analysis by nanoelectrospray ionization mass spectrometry.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Solvent | Cleansing agent
| SKU | 02194002-CF |
| Alternate Names | 1,1,1-Trichloromethane; Trichloromethane; Methylidyne trichloride; Triclorometano; Formyl trichloride |
| Application Notes | Chloroform is use as a solvent for fats, oils, rubber, alkaloids, waxes, gutta-percha, resins; as cleansing agent; in fire extinguishers to lower the freezing temperature of carbon tetrachloride. Procedures have been described for the use of chloroform in lambda plaques storage, lambda cDNA storage, the removal of mineral oil from PCR reaction samples, oligonucleotide purification, a hydroxyl radical footprinting protocol, a transcriptional run-on assay protocol, and an overlay assay for beta-galactosidase activity. It has also been used with phenol in such procedures as DNA recovery from polyacrylamide gels, ethidium bromide removal from DNA preparations, lysis protocols for plasmid DNA isolation, RNase removal, and purification of yeast DNA. A protocol describes the use of chloroform in a high-performance thin-layer chromatography protocol for sphingomyelin analysis. Chloroform can also be used in chloroform/methanol mixtures for the isolation of cardiolipids from Geobacillus stearothermophilus and their subsequent MS analysis. The isolation of the bacteriocin amylovorin L471 from Lactobacillus amylovorus DCE 471 in culture broth has been reported, using chloroform/methanol extraction and precipitation in the procedure. Chloroform/2-butanol mixtures can be used for the extraction of steroid sulfates for analysis by nanoelectrospray ionization mass spectrometry. |
| Auto Ignition | Not flammable (USCG, 1999) |
| Base Catalog Number | 194002 |
| Beilstein Registry Number | 1731042 |
| Boiling Point | 143° F at 760 mm Hg (EPA, 1998) |
| CAS # | 67-66-3 |
| Density | 1.4832 at 68° F (EPA, 1998) |
| EC Number | 200-663-8 |
| Flash Point | None (EPA, 1998) |
| Grade | Molecular Biology Grade |
| Hazard Statements | H302-H315-H351-H373 |
| Melting Point | -82.3° F (EPA, 1998) |
| Molecular Formula | CHCl3 |
| Molecular Weight | 119.369 g/mol |
| Personal Protective Equipment | Eyeshields, Faceshields, full-face respirator , Gloves, multi-purpose combination respirator cartridge |
| Purity | ≥99% |
| Refractive Index | n20/D 1.445(Lit.) |
| RTECS Number | FS9100000 |
| Safety Symbol | GHS07, GHS08 |
| Solubility | less than 1 mg/mL at 66° F (NTP, 1992) |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| Vapor Density | 4.12 (EPA, 1998) (Relative to Air) |
| Vapor Pressure | 100 mm Hg at 50.72° F (EPA, 1998) |
![MP COA [194002] lot ID: 1133J](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [194002] lot ID: 1133J")