Dimethyl Sulfoxide
Application Notes
Dimethyl Sulfoxide is used to enhance dermal absorption of many chemicals, as a solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers, in antifreeze or hydraulic fluids, as a cryopreservative for cell cultures, oxidation of thiols and disulfides to sulfonic acids, as a PCR cosolvent to help improve yields, especially in long PCR. DMSO is routinely used in polymerase chan reaction (PCR), amplification of cDNA libraries, DNA sequencing, column-loading buffers for poly(A)+ RNA selection, buffers for the transformation of competent E. coli, and transfection protocols.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
PCR Cosolvent
| SKU | 02194819-CF |
| Alternate Names | DMSO; Methyl sulfoxide; Methyl sulphoxide; Sulfinylbismethane |
| Application Notes | Dimethyl Sulfoxide is used to enhance dermal absorption of many chemicals, as a solvent for many organic and inorganic compounds including fats, carbohydrates, dyes, resins, and polymers, in antifreeze or hydraulic fluids, as a cryopreservative for cell cultures, oxidation of thiols and disulfides to sulfonic acids, as a PCR cosolvent to help improve yields, especially in long PCR. DMSO is routinely used in polymerase chan reaction (PCR), amplification of cDNA libraries, DNA sequencing, column-loading buffers for poly(A)+ RNA selection, buffers for the transformation of competent E. coli, and transfection protocols. |
| Auto Ignition | 419° F (USCG, 1999) |
| Base Catalog Number | 194819 |
| Beilstein Registry Number | 506008 |
| Boiling Point | 372° F at 760 mm Hg (NTP, 1992) |
| CAS # | 67-68-5 |
| Density | 1.101 at 68° F (USCG, 1999) |
| Dielectric Constant | 45 (Lit.) |
| EC Number | 200-664-3 |
| Flash Point | 203° F (NTP, 1992) |
| Format | Liquid |
| Grade | Molecular Biology Grade |
| Melting Point | 65.3° F (NTP, 1992) |
| Molecular Formula | C2H6OS |
| Molecular Weight | 78.129 g/mol |
| Personal Protective Equipment | Eyeshields, Full-face respirator, Gloves, Multi-purpose combination respirator cartridge, Respirator filter |
| Refractive Index | n20D 1.479 (lit.) |
| RTECS Number | PV6210000 |
| Solubility | greater than or equal to 100 mg/mL at 68° F (NTP, 1992) |
| Sterilization of Solutions | To prepare sterile solutions use a teflon or nylon membrane to sterile-filter the DMSO - do not use a cellulose acetate membrane. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| Vapor Density | 2.71 (NTP, 1992) (Relative to Air) |
| Vapor Pressure | 0.42 mm Hg at 68° F (NTP, 1992) |
![MP COA [194819] lot ID: 1828A](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [194819] lot ID: 1828A")
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