2-Mercaptoethanol
Application Notes
2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Purification | Reducing agent
| SKU | 02194834-CF |
| Alternate Names | 2-Thioethanol; 2-Hydroxyethylmercaptan; 2-Hydroxy-1-Ethanethiol; Monothioethyleneglycol; β-Mercaptoethanol; BME; Thioethylene glycol; 2-Hydroxyethyl Mercaptan; 1-ethane-2-thiol; 2-ME; 2-thioethanol |
| Application Notes | 2-Mercaptoethanol is used to reduce disulfide linkages in solubilizing proteins for gel electrophoresis (typically used in SDS-PAGE sample buffer at 5% concentration). Also reduces excess oxidative polymerization of catalysts. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure. In solution, 2-mercaptoethanol is readily oxidized in air to a disulfide, especially at alkaline pH. Because of this property, it is widely used to protect proteins, enzymes in particular, from becoming inactive. An excess of 2-mercaptoethanol (generally used at 0.01 M) will maintain the protein thiol groups in their reduced state. |
| Auto Ignition | 295°C |
| Base Catalog Number | 194834 |
| Beilstein Registry Number | 773648 |
| Boiling Point | 315 to 316° F at 742 mm Hg (decomposes) (NTP, 1992) |
| CAS # | 60-24-2 |
| Density | 1.1143 at 68° F (NTP, 1992) |
| EC Number | 200-464-6 |
| Flash Point | 165° F (NFPA, 2010) |
| Format | Liquid |
| Grade | Molecular Biology Grade |
| Hazard Statements | H301-H310 + H330-H315-H317-H318-H410 |
| Melting Point | -148° F (NTP, 1992) |
| Molecular Formula | C2H6OS |
| Molecular Weight | 78.129 g/mol |
| Personal Protective Equipment | Faceshields, Full-face respirator, Gloves, Goggles, Multi-purpose combination respirator cartridge |
| Purity | ≥98% |
| Refractive Index | n20/D 1.500(lit.) |
| RTECS Number | KL5600000 |
| Safety Symbol | GHS05, GHS06, GHS09 |
| Solubility | greater than or equal to 100 mg/mL at 68° F (NTP, 1992) |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
| Vapor Density | 2.69 (NTP, 1992) (Relative to Air) |
| Vapor Pressure | 1 mm Hg at 68° F (NTP, 1992) |
![MP COA [194834] lot ID: 2156A](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [194834] lot ID: 2156A")