Sodium Bicarbonate
Application Notes
Sodium bicarbonate is broadly used as a physiological buffer in cell culture applications. The pKa for bicarbonate <-> carbonic acid reaction is 6.4 The pKa for bicarbonate <-> carbonate reaction is 10.3. Sodum bicarbonate has two pKas, 6.4 and 10.3. The compound can react with both acids and bases, therefore it can be used as a neutralizer. Sodium bicarbonate may be used to remove or wash acidic impurities from a crude liquid to yield a purer sample. Sodium bicarbonate reacts with the carboxyl groups to give a fast-forming and effervescent CO2 formation which has been used to test for the presence of carboxylic groups in proteins.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Biological Buffers | Buffers | Cell Biology | Cell Culture | Cell Signaling | Laboratory pH neutralizer that reacts with acids and bases
| SKU | 02195497-CF |
| Alternate Names | Sodium Hydrogen Carbonate; Baking Soda; Carbonic acid monosodium salt; Monosodium carbonate |
| Application Notes | Sodium bicarbonate is broadly used as a physiological buffer in cell culture applications. The pKa for bicarbonate <-> carbonic acid reaction is 6.4 The pKa for bicarbonate <-> carbonate reaction is 10.3. Sodum bicarbonate has two pKas, 6.4 and 10.3. The compound can react with both acids and bases, therefore it can be used as a neutralizer. Sodium bicarbonate may be used to remove or wash acidic impurities from a crude liquid to yield a purer sample. Sodium bicarbonate reacts with the carboxyl groups to give a fast-forming and effervescent CO2 formation which has been used to test for the presence of carboxylic groups in proteins. |
| Base Catalog Number | 195497 |
| Beilstein Registry Number | 4153970 |
| CAS # | 144-55-8 |
| Density | 2.159 (NTP, 1992) |
| EC Number | 205-633-8 |
| Format | Powder |
| Hazard Statements | H303 |
| Melting Point | Decomposes at 228° F (NTP, 1992) |
| Molecular Formula | NaHCO3 |
| Molecular Weight | 84.006 g/mol |
| Personal Protective Equipment | Eyeshields, Gloves, Respirator filter |
| pKa | (1) 6.37, (2) 10.25 (carbonic acid)(Lit.) |
| Purity | ≥99.5% |
| Refractive Index | 1.500(Lit.) |
| RTECS Number | VZ0950000 |
| Solubility | 1 in 10 % at 77° F (NTP, 1992) |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [195497] lot ID: 1701A](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [195497] lot ID: 1701A")