DL-二硫苏糖醇(DTT),DL-Dithiothreitol

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Key features and details

Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE
Useful for stabilizing sulfhydryl containing enzymes
Less pungent and is less toxic than 2-mercaptoethanol

SKU: 04856126-CF

Synonyms

1,4-Dithiothreitol; DTT; 1,4-Dithio-DL-threitol; Cleland Reagent; (±)-threo-1,4-Dimercapto-2,3-butanediol

CAS Number:

27565-41-9

Molecular Formula:

C4H10O2S2

Beilstein Registry Number:

1719757

EC Number:

222-468-7

MDL Number:

MFCD00004877

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Product Description

Dl-Dithiothreitol

Application Notes

Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Sulfhydryl reducing agent | Blotting | Protein Purification

Specifications

SKU	04856126-CF
Alternate Names	1,4-Dithiothreitol; DTT; 1,4-Dithio-DL-threitol; Cleland Reagent; (±)-threo-1,4-Dimercapto-2,3-butanediol
Application Notes	Dithiothreitol is useful for stabilizing sulfhydryl containing enzymes. Effective in sample buffers for reducing protein disulfide bonds prior to SDS-PAGE. DTT can also be used for reducing the disulfide bridge of the cross-linker N,N'-bis(acryloyl)cystamine to break apart the matrix of a polyacrylamide gel. DTT is less pungent and is less toxic than 2-mercaptoethanol.
Base Catalog Number	856126
Beilstein Registry Number	1719757
CAS #	27565-41-9
EC Number	222-468-7
Format	Powder
Grade	Electrophoresis grade
Half Life	1.4( pH 8.5 at 2 °C)
Hazard Statements	H302-H315-H319-H335
Molecular Formula	C4H10O2S2
Personal Protective Equipment	Dust mask , Eyeshields, Faceshields, Gloves
Purity	≥99.5%
RTECS Number	EK1610000
Safety Symbol	GHS07
Typical Working Concentration	Typically, a seven fold lower concentration of DTT (100 mM) is needed than is used for 2-mercaptoethanol (5% v/v, 700 mM).
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.