Horseradish peroxidase (HRP) is isolated from horseradish roots and belongs to the ferroprotoporphyrin group of peroxidases. HRP is a single chain polypeptide containing four disulfide bridges. It is a glycoprotein containing 18% carbohydrate. The carbohydrate composition consists of galactose, arabinose, xylose, fucose, mannose, mannosamine, and galactosamine depending upon the specific isozyme.
Application Notes
Horseradish peroxidase is used for production of peroxidase conjugated antisera. It is a widely used label for immunoglobulins in many different immunochemistry applications including ELISA, immunoblotting, and immunohistochemistry. HRP can be conjugated to antibodies by several different methods including glutaraldehyde, periodate oxidation, through disulfide bonds, and also via amino and thiol directed crosslinkers. HRP is the most desired label for antibodies, since it is the smallest and most stable of the three most popular enzyme labels (HRP, β-galactosidase, and alkaline phosphatase) and its glycosylation leads to lower non-specific binding.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Protein Labeling | Production of peroxidase conjugated antisera | Immunoblot | ELISA | Immunohistochemistry
| SKU | 0836451-CF |
| Alternate Names | Horseradish peroxidase |
| Application Notes | Horseradish peroxidase is used for production of peroxidase conjugated antisera. It is a widely used label for immunoglobulins in many different immunochemistry applications including ELISA, immunoblotting, and immunohistochemistry. HRP can be conjugated to antibodies by several different methods including glutaraldehyde, periodate oxidation, through disulfide bonds, and also via amino and thiol directed crosslinkers. HRP is the most desired label for antibodies, since it is the smallest and most stable of the three most popular enzyme labels (HRP, β-galactosidase, and alkaline phosphatase) and its glycosylation leads to lower non-specific binding. |
| Base Catalog Number | 364511,364514 |
| CAS # | 9003-99-0 |
| EC Number | 232-668-6 |
| Formulation Details | Powder |
| Isoelectric Point | Isozymes range from 3.0–9.0 |
| Protein or Enzyme Type | Other Proteins, Enzymes Peptides |
| Solubility | Soluble in distilled water or dilute buffer. |
| Source | Horseradish Roots |
| Specific Activity | ≥200 units/mg |
| Unit Definition | The amount of enzyme causing the production of one milligram of purpurgallin in 20 seconds at 25 °C under the given assay conditions. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
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