Name: IgG Rabbit Anti-Sheep Red Blood Cells, 2 mL
SKU: 0855806

Product is the lyophilized powder of rabbit IgG fraction to sheep red blood cells and buffer salts.

Application Notes

Immunoblotting; Immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. This product is suitable for sensitizing cells in hemolytic complement assays, and for lymphocyte screening procedures, especially screening for T cells and T receptor-bearing lymphocytes. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Immunoassays | immmunoelectrophoresis| Immunohistochemistry (Paraffin; acetone)

SKU	0855806
Antibody Type	Polyclonal Antibody
Application Notes	Immunoblotting; Immunostaining of acetone-fixed frozen and formalin-fixed, paraffin-embedded tissue sections. This product is suitable for sensitizing cells in hemolytic complement assays, and for lymphocyte screening procedures, especially screening for T cells and T receptor-bearing lymphocytes. This product is suitable for conjugation with enzymes, radiolabels, fluorochromes or other markers.
Base Catalog Number	55806
Concentration	Total Protein: 20.5 mg/mL Hemagglutination Titer: 1:1600
Conjugate	Unconjugated
Format	lyophilized
Formulation Details	Lyophilized
Grade	Molecular Grade
Host	Rabbit
Pack Size	2 mL
Preparation Method	The antiserum is prepared by immunizing healthy rabbits with red blood cells obtained from healthy sheep. The IgG fraction is prepared from the specific rabbit antiserum by delipidation, ammonium sulfate fractionation and anion exchange chromatography. The resulting IgG fraction is dialyzed into 0.02M sodium phosphate, 0.14M sodium chloride, pH 7.3, 0.05% sodium azide, adjusted to standard titer, filtered through a 0.22 µm filter, vialed and lyophilized.
Solubility	Reconstitution: Reconstitute product with 2.0 mL of deionized or distilled water. Gentle swirling may be used to speed rehydration. Avoid vigorous shaking of the reconstituted material
Species Reactivity	Sheep
Specificity	The total protein is measured using the Biuret procedure; with bovine albumin as a standard. Hemagglutination titer is standardized with an in-house control by measurement of agglutination end-point with 10% sheep red blood cells. Each IgG fraction is tested for purity at a minimum of 40 mg/ml using immunoelectrophoresis. The product is mostly rabbit IgG; no trace of albumin is detected.
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.