Anti-Sheep
Host: dog
Form: lyophilized antiserum
Applications: Positive Coombs' Test Control
Because anti-globulin positive canine red blood cells are unstable, MP offers dog antiserum to sheep red blood cells. This reagent may be used to sensitize fresh sheep red blood cells for use as a positive control. It may also be used to prepare red blood cells for the canine Rose-Waller procedure.
Application Notes
Dog anti-sheep red blood cells can be used to sensitize sheep red blood cells (RBCs) for use in either a canine Rose-Waaler test, or in canine antiglobulin test as a positive control.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays
| SKU | 08646451 |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Dog anti-sheep red blood cells can be used to sensitize sheep red blood cells (RBCs) for use in either a canine Rose-Waaler test, or in canine antiglobulin test as a positive control. |
| Base Catalog Number | 646451 |
| Conjugate | Unconjugated |
| Format | lyophilized |
| Formulation Details | Lyophilized |
| Grade | Molecular Grade |
| Host | Dog |
| Pack Size | 1 mL |
| Solubility | Soluble in water |
| Species Reactivity | Sheep |
| Specificity | Antiserum to sheep red blood cells is produced by immunization of dogs with sheep RBCs. The harvested antiserum is incubated at 60°C for 30 minutes to inactivate complement. The antiserum is tested to determine its agglutination titer and the sub-agglutination titer for sensitization of sheep RBCs in canine antiglobulin or Rose-Waaler procedures. The optimum dilution for sensitization of sheep RBCs in these procedures will vary from one laboratory to another. A sub-agglutinating dose is calculated for each lot of antiserum (see below). Please note that with some sheep RBC preparations; the sub-agglutinating dose may be as high as 1:500 p 1:2;000; even though we my report a much lower titer with our RBC preparation. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
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