Nutrient Broth is a general purpose medium used for cultivating a broad variety of fastidious and non-fastidious microorganisms with non-exacting nutritional requirements. Peptone and yeast extract provide nitrogenous compounds, vitamin B complex, amino acids and other essential growth nutrients. Sodium chloride is used to maintain the osmotic balance and glucose is the fermentable carbohydrate source of the media.
Application Notes
Nutrient Broth is used in many laboratory procedures as is, or with added indicators, carbohydrates, organic liquids, salts, etc. This medium is used in accordance with official recommended procedures for the bacteriological analyses of water, milk and dairy products, in foods of important sanitation, tests for sensitivity and resistance and as a base to prepare media supplemented with other nutrients. It can be used for enumeration, isolation and enrichment as well as providing a high grade base for the preparation of special media. With the addition of blood, ascites fluid or serum, they can also be used to cultivate streptococci, pneumococci and erysipelas species etc.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Cell Culture | Cell growth supplement
| SKU | 091007917 |
| Alternate Names | Standard - Nutrient Broth |
| Application Notes | Nutrient Broth is used in many laboratory procedures as is, or with added indicators, carbohydrates, organic liquids, salts, etc. This medium is used in accordance with official recommended procedures for the bacteriological analyses of water, milk and dairy products, in foods of important sanitation, tests for sensitivity and resistance and as a base to prepare media supplemented with other nutrients. It can be used for enumeration, isolation and enrichment as well as providing a high grade base for the preparation of special media. With the addition of blood, ascites fluid or serum, they can also be used to cultivate streptococci, pneumococci and erysipelas species etc. |
| Base Catalog Number | 1007917 |
| Format | Powder |
| Formulation Details | Powder | Nutrient Broth is a liquid medium; produced according to the formula from APHA and AOAC. Approximate formula: Gelatin Peptone: 5.0 g/L; Beef Extract: 3.0 g/L; Final pH: 6.9 +/- 0.1 |
| Media Type | Nutrient Broth |
| Pack Size | 500 g |
| pH | 6.7 - 7.1 (8 g/L) |
| Preparation Method | Dissolve 8 g of the medium in a liter of deionized or distilled water. Dispense as desired and sterilize at 121 °C (15 lbs. of pressure) for 15 minutes. |
| Solubility | Soluble in water (8 g/L). |
| Sterility | Sterilize at 121 °C (15 lbs. of pressure) for 15 minutes. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [1007917] lot ID: 1231F](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [1007917] lot ID: 1231F")