Minimum Essential Medium Eagle with Earle's Salts and 2.0 g/L sodium bicarbonate w/o L-glutamine, L-cysteine, L-cystine, and L-methionine, 500 mL

MEM, originally prepared by Harry Eagle, is one of the most popular cell culture media. Upon his attempts to cultivate normal mammalian fibroblasts and certain HeLa cell subtypes, it was revealed that the nutritional needs of these cell types could not be met by BME. Further studies led to the development of MEM incorporating specific modifications such as higher amino acid concentrations for the cultivation of fastidious cells. MP's MEM may be used to support the growth of cells in monolayers, in suspension and wide variety of other cell types with proper supplementation. MP offers MEM with either Earle's or Hanks' salts.

Application Notes

Minimum Essential Medium Eagle With Earle's Salts and 2.0 g/L Sodium Bicarbonate Without L-Glutamine, L-Cysteine, L-Cystine, and L-Methionine is generally used to grow attached cell lines, such as fibroblasts, in the presence of FBS, calf or horse sera. It may be used to support the growth of cells in monolayers, in suspension and wide variety of other cell types with proper supplementation. It is a commonly used deficient media for metabolic labelling procedures.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Mammalian Cell Culture | Used to grow attached cell lines; such as fibroblasts; in the presence of FBS; calf or horse sera