Trypan Blue is a blue acid dye with a strong affinity for cellulose containing substrates such as cotton; less affinity for proteinaceous materials. Trypan blue solution may be used in trypan blue based cytotoxitiy and proliferation assays. Trypan Blue is used as a vital dye which is especially important because it is taken up by the reticuloendothelial system. Clark describes assays for the study of teratogenic action of trypan blue on embryonic tissues using Davis and Sauter's fluorescence method and for the staining of collagen, including very fine fibrils, muscle and cornified epithelium using the Van Gieson method. Trypan blue is also recommended for use in dye exclusion procedures for viable cell counting. Non-viable cells will up-take trypan blue at a faster rate than viable cells.
Application Notes
Trypan Blue is used as a vital dye which is especially important because it is taken up by the reticuloendothelial system. Clark describes assays for the study of teratogenic action of trypan blue on embryonic tissues using Davis and Sauter's fluorescence method and for the staining of collagen, including very fine fibrils, muscle and cornified epithelium using the Van Gieson method. Trypan blue is also recommended for use in dye exclusion procedures for viable cell counting. Non-viable cells will up-take trypan blue at a faster rate than viable cells.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Cell Counting; Cell Viability; Cryopreservation | Cytotoxitiy and proliferation assays
| SKU | 091691049 |
| Alternate Names | Direct Blue 14; Niagara Blue 3B; Chlorazol blue 3B; Benzo blue 3B; Dianil blue H3G; Congo blue 3B; Naphthamine blue 3BX; Benzamine blue 3B; Azidine blue 3B; Diamine Blue 3B; |
| Application Notes | Trypan Blue is used as a vital dye which is especially important because it is taken up by the reticuloendothelial system. Clark describes assays for the study of teratogenic action of trypan blue on embryonic tissues using Davis and Sauter's fluorescence method and for the staining of collagen, including very fine fibrils, muscle and cornified epithelium using the Van Gieson method. Trypan blue is also recommended for use in dye exclusion procedures for viable cell counting. Non-viable cells will up-take trypan blue at a faster rate than viable cells. |
| Base Catalog Number | 1691049 |
| CAS # | 72-57-1 |
| Concentration | 0.4% (w/v) Solution |
| Format | Solution |
| Formulation Details | Liquid | Potassium Phosphate Dibasic: 600.00000 mg/L; Sodium Chloride [NaCl]: 8100.00000 mg/L; Trypan Blue: 4000.00000 mg/L |
| Molecular Formula | C34H24N6Na4O14S4 |
| Molecular Weight | 960.82 |
| Pack Size | 100 mL |
| pH | 7.0 - 8.0 |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [1691049] lot ID: 16910002](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [1691049] lot ID: 16910002")
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