Geneclean III DNA纯化试剂盒, 600 Preps

Key features and details

For the rapid purification of DNA from TAE and TBE agarose gels
Optimal purification of 200 bp to 20 kb DNA
Each spin column can bind up to 10 μg of DNA
Kit can also be used to purify DNA from polyacrylamide gels

SKU: 111001600

Synonyms

DNA Clean-Up Kit

Sample Volume:

Up to ~0.5 ml gel slice by volume or ~1.0 ml solution

Packaging size:

600 Preps

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Product Description

The GENECLEAN® III kit simplifies the process of purifying DNA into three easy steps: Bind,Wash and Elute. Ethanol precipitation is never required and purified DNA is immediately ready for a wide variety of downstream applications.

Application Notes

The GENECLEAN® III Kit is used to purify fragments of DNA from 200 bp to 20 kb from solutions and agarose gels. Examples of applications are desalting, elimination of proteins from enzymatic reactions, removal of primers and unincorporated nucleotides from enzymatic reactions, separation of linearized from uncut vector and isolation of PCR products away from genomic DNA.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Purify DNA from agarose gel| Desalting| Elimination of proteins from enzymatic reactions| Removal of primers and unincorporated nucleotides from enzymatic reactions| Separation of linearized from uncut vector| Isolation of PCR products away from genomic DNA

Specifications

SKU	111001600
Alternate Names	DNA Clean-Up Kit
Application Notes	The GENECLEAN® III Kit is used to purify fragments of DNA from 200 bp to 20 kb from solutions and agarose gels. Examples of applications are desalting, elimination of proteins from enzymatic reactions, removal of primers and unincorporated nucleotides from enzymatic reactions, separation of linearized from uncut vector and isolation of PCR products away from genomic DNA.
Base Catalog Number	1001600
Brand	GENECLEAN®
Components	NaI\| New wash concentrate\| TBE modifier\| EZ-Glassmilk™\| Label Block\| Elution Solution
Format	Geneclean® slurry
Pack Size	600 Preps
Personal Protective Equipment	Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
pH	Above 500 bp: 4.5 M NaI pH 7.0 - 7.4\| Between 200 - 500 bp: 4.5 M NaI pH 6.0\| NEW Wash: pH Range: 7.4 - 8.0\| TBE Modifier: pH 5.8 ± 0.2
Preparation Method	Pour contents of NEW Wash Concentrate (14 ml) into a bottle that accommodates at least 650 ml of liquid. Add 280 ml of distilled water and mix well. Add 310 ml of 100% (200 proof) ethanol and mix well. Label the prepared NEW Wash bottle with the label provided. Shake Glassmilk™ bottle until silica particles are in a uniform suspension.
Recommended Alternate	GENECLEAN® SPIN Kit
Safety Symbol	GHS05, GHS06
Sample Volume	Up to ~0.5 ml gel slice by volume or ~1.0 ml solution
Unit Definition	One µL of EZ-GLASSMILK™ will bind with one µg of DNA in 4.5 M Nal in 5 minutes at room temperature.
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.