MERmaid® SPIN Kit is designed for rapid purification of small MW single-stranded or double-stranded DNA (10 bp to 200 bp) from agarose or polyacrylamide gels, nucleotides, salts, proteins, or other undesirable components of a mixture. This kit combines the advantages of silica binding with a convenient spin column format, eliminating the need for expensive resins or toxic organic compounds such as phenol and chloroform.
Application Notes
The MERmaid SPIN Kit is designed for purifying small MW DNA away from gel material, nucleotides, salts, proteins, or other undesirable components of a mixture. Single-stranded or double-stranded DNA oligomers or PCR products of 10-200 nucleotides in length are readily purified from agarose and polyacrylamide gels or from solutions. Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Rapid purification of DNA
| SKU | 111105200-CF |
| Alternate Names | Purification of small molecular weight DNA |
| Application Notes | The MERmaid SPIN Kit is designed for purifying small MW DNA away from gel material, nucleotides, salts, proteins, or other undesirable components of a mixture. Single-stranded or double-stranded DNA oligomers or PCR products of 10-200 nucleotides in length are readily purified from agarose and polyacrylamide gels or from solutions. Purified DNA can be used in enzymatic reactions, conventional or automated sequencing, cloning and microarray analysis. |
| Base Catalog Number | 1105200 |
| Brand | MERmaid™ |
| Components | GLASSFOG™ SPIN Bind Solution |MERmaid™ SPIN High Salt Wash |MERmaid™ SPIN Ethanol Wash Concentrate |MERmaid™Elution Solution |50x Electrophoresis Buffer |BioGel™ |MERmaid™ SPIN Filters |Elution Catch Tubes |Label Block. |
| Format | GLASSFOG™ slurry plus SPIN filter |
| Personal Protective Equipment | Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice. |
| Preparation Method | Before first use, add the 22.5 mL of 200 proof (100% ethanol) to the MERmaid™ SPIN Ethanol Wash Concentrate (2.5 mL) and mix well. Do not use denatured alcohol because it can cause precipitation of salts. Label the container. Store tightly capped at 15°-30°C. |
| Safety Symbol | GHS07 |
| Sample Source | Gels and Solutions |
| Sample Type | Enzymatic Reactions, Solutions, Agarose Gels, Polyacrylamide Gels |
| Sample Volume | Up to ~300 mg gel slice or up to ~300 µl DNA solution |
| Unit Definition | Each prep will bind up to 10 μg of either ssDNA or dsDNA. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [1105200] lot ID: 118040](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [1105200] lot ID: 118040")
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