The EZ-Yeast™ Transformation Kit is designed for high-throughput transformation with efficiencies up to 103 transformants per μg plasmid DNA. Perfect for a quick analysis of potentially positive two-hybrid colonies, the EZ-Yeast™ Transformation Kit does not require fully competent cells prior to transformation. Simply remove a 3-4 mm colony or 0.5 mL overnight culture to a new tube, add vector and carrier DNA, incubate for 30 minutes and plate on selective media.
Application Notes
The EZ-Yeast Transformation Kit is designed for high throughput transformations. It is ideal for cases where a large number of transformations must be performed, but only a few transformants are needed.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Transformation of yeast cells
SKU | 112100200 |
Alternate Names | Yeast 2-hybrid reporter kit |
Application Notes | The EZ-Yeast Transformation Kit is designed for high throughput transformations. It is ideal for cases where a large number of transformations must be performed, but only a few transformants are needed. |
Base Catalog Number | 2100200 |
Components | EZ-Transformation Solution |Carrier DNA |
Format | Single Reagent Kit plus DNA |
Pack Size | 200 Preps |
Personal Protective Equipment | Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice. |
Recommended Alternate | Alkali-Cation™ Yeast Transformation Kit |
Sample Type | Overnight yeast culture or colonies from plates |
Sample Volume | 500 µL or 3-4 mm colony |
Unit Definition | ≤ 103 transformants/µg plasmid DNA |
Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |