Super Broth, capsules, 1 kg

Name: Super Broth, capsules, 1 kg
SKU: 113010031

Key features and details

Super Broth is a robust L-Broth variant, nutritionally rich medium developed for growth and maintenance of recombinant E. coli strains and generates higher plasmid densities. It has the formulation of 35 g Tryptone, 20 g Yeast Extract, 5 g NaCl. Encapsulated Media: simply count out the appropriate number of capsules and drop them in water and autoclave.

SKU: 113010031

Synonyms

Bacteriophage Media

Packaging size:

1 kg

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Product Description

SuperBroth is enriched medium with high levels of tryptone and yeast extract. Due to the high levels of peptides, amino acids, vitamins and cofactors high yields of E. coli cells and lambda bacteriophages are obtained.

Application Notes

Superbroth is used for obtaining high yields of lambda phage and phagemids in E. coli.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Specifications

SKU	113010031
Alternate Names	Bacteriophage Media
Application Notes	Superbroth is used for obtaining high yields of lambda phage and phagemids in E. coli.
Base Catalog Number	3010031
Boiling Point	1461 °C (2662 °F)(Lit.)
Components	35 g tryptone, 20 g yeast extract, 5 g NaCl
Format	Capsules
Formulation Details	Contents Per Liter: 35 g Tryptone-B; 20 g Yeast Extract; 5 g NaCI.
Grade	Molecular Biology Certified™
Media Type	Super Broth
Pack Size	1 kg
Personal Protective Equipment	Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
pH	Acceptable pH range is 6.6 ± 0.2 at 25°C.
Preparation Method	Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of lambda phage and phagemids in E. coli when combined in SuperBroth formulations. Chosen reagents are combined in the proper proportions according to the Superbroth formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation was used to prepare liquid medium by adding 60 g per liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve and autoclaved at 121 °C for 15 minutes. Autoclaving at higher temperatures or for longer periods will result in darkening of the sterile medium, (a darker color does not noticeably affect growth of cells, only the appearance of the sterile medium).
Solubility	Soluble in water (60 g/L).
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.