4+ dropout formulation (without L-Histidine, L-Leucine, L-Tryptophan and Uracil) of Synthetic Complete Supplement Mixture (SC) of amino acids for S. cerevisiae growth media. Also known as Hopkins Mixture and is available in complete and dropout formulations.
Application Notes
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. This SC-Dropout medium create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture
| SKU | 114440022 |
| Alternate Names | Synthetic Complete Supplement Mixture; Hopkins Mixture |
| Application Notes | The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. This SC-Dropout medium create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs. |
| Base Catalog Number | 4440022 |
| Format | Powder |
| Formulation Details | Contents per Liter: Adenine 21 mg; L-Alanine 86.5 mg; L-Arginine HCl 85.6 mg; L-Asparagine 85.6 mg; L-Aspartic Acid 85.6 mg; L-Cysteine HCl 85.6 mg; Glutamine 85.6 mg; L-Glutamic Acid 85.6 mg; Glycine 85.6 mg; L-Histidine HCl 0 mg; Myo-Inositol 85.6 mg; L-Isoleucine 85.6 mg; L-Leucine 0 mg; L-Lysine HCl 85.6 mg; L-Methionine 85.6 mg; Para-Aminobenzoic Acid 8.6 mg; L-Phenylalanine 85.6 mg; L-Proline 85.6 mg; L-Serine 85.6 mg; L-Threonine 85.6 mg; L-Tryptophan 0 mg; L-Tyrosine 85.6 mg; Uracil 0 mg; L-Valine 85.6 mg; Total 2000.6 mg |
| Grade | Molecular Biology Certified™ |
| Media Type | Yeast SC (synthetic complete) |
| Pack Size | 30 g |
| Personal Protective Equipment | Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice |
| pH | pH Range (with DOB, after autoclave): 4.0 - 4.8 at 25 °C |
| Preparation Method | Reagents are tested and chosen for their ability to promote optimum growth of yeast strains when combined in the Drop Out Base (DOB) or Drop Out Base with 17 g Agar (DOBA) formula, with SC or SC-dropout formulations. DOB contains 1.7g Yeast Nitrogen Base, 5g Ammonium Sulfate, and 20g Dextrose per liter. Chosen reagents are combined in the proper proportions according to the formulation listed on the Certificate of Analysis and milled to thoroughly blend the reagents for a uniform distribution. A sample of the milled and blended formulation was used to prepare liquid medium by adding 43.7g DOBA powder and 2 g of SC to a liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve the dextrose and autoclaved at 121 °C for 15 minutes. After cooling to 50 °C, plates were poured. DOBA with SC liquid solution was visually inspected after autoclaving and pouring plates for complete dissolution of components. Solid plates were inoculated with control S.cerevisiae yeast strain and incubated for 3 days at 30 °C. Colony size was compared to the same strain grown on standard lot test media (SLTM). |
| Solubility | Soluble in water. |
| Sterilization of Solutions | Autoclave at 121 °C for 15 minutes |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP SDS [4440022]](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP SDS [4440022]")