Single dropout formulation (without Leucine) of Synthetic Defined (SDA) Yeast Media with agar includes all necessary components for selective growth of Saccharomyces cerevisiae.
Application Notes
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. This CSM-Dropout medium create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Cell culture |Proteomics |Purification and Detection |Protein Analysis |Yeast Culture |Plasmid Production |Protein Expression |Gene Expression |Cloning |Gene Function |Gene Regulation |Automated Bacterial Growth Media |S. cerevisiae Culture
| SKU | 114811165-CF |
| Alternate Names | synthetic defined medium without leucine |
| Application Notes | The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. This CSM-Dropout medium create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs. |
| Base Catalog Number | 4811165 |
| Format | Pouch |
| Formulation Details | Contents per Liter: 1.7 g YNB |5 g Ammonium Sulfate |20 g Dextrose |0.69 g CSM-Leu |17 g Agar-Y |
| Grade | Molecular Biology Certified™ |
| Media Type | Yeast Media |
| Personal Protective Equipment | Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: PVC or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice |
| pH | pH Range (with DOB, after autoclave): 4.0 - 4.8 at 25 °C |
| Preparation Method | Reagents are tested and chosen for their ability to promote optimum growth of yeast strains when combined in the Synthetic Defined (SD) or Synthetic Defined Agar (SDA) or SD/SDA-Dropout formula. SD is 1.7 g Yeast Nitrogen Base, 5 g Ammonium Sulfate and 20g Dextrose per liter. SDA is SD with 17% agar. Chosen reagents are combined in the proper proportions according to the formulation listed in the certificate of analysis and milled to a powder which thoroughly blends the reagents for uniform distribution. A sample of the milled and blended formulation was used to prepare liquid medium by adding 27.49 SD powder or the appropriate weight ofSD-Dropout to a liter of purified water. The combinations were mixed by hand for about 1 minute to dissolve the dextrose and autoclaved at 121 °C for 25 minutes. |
| Solubility | Soluble in water. |
| Sterilization of Solutions | Autoclave at 121 °C for 15 minutes. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
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