Isolation of total RNA from soil supernatants The FastRNA™ Pro Soil-Indirect Kit is designed to efficiently isolate total RNA from organic material found in soil supernatants. Cellular material is washed away from soil particles, centrifuged, and homogenized by any FastPrep® Instrument in impact-resistant 2 mL tubes of Lysing Matrix E. Total RNA is released into a protective solution, extracted, precipitated, and cleaned with the proprietary RNAMATRIX™ and a Quick-Clean Spin Filter. Total RNA prepared with this kit is suitable for RT-PCR analysis and other downstream applications.
Application Notes
FastRNA™ Pro Soil-Indirect Kit is used to isolate total RNA from organic material found in soil samples. This kit allows for the sample to be slurried with water or buffer in order to further separate biomass from the soil or environmental matrix, and the resulting supernatant is then filtered, lysed, and purified through the rest of the protocol. Total RNA isolated is suitable for RT-PCR analysis and other downstream applications.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Isolation of RNA from soil samples
| SKU | 116075050 |
| Application Notes | FastRNA™ Pro Soil-Indirect Kit is used to isolate total RNA from organic material found in soil samples. This kit allows for the sample to be slurried with water or buffer in order to further separate biomass from the soil or environmental matrix, and the resulting supernatant is then filtered, lysed, and purified through the rest of the protocol. Total RNA isolated is suitable for RT-PCR analysis and other downstream applications. |
| Base Catalog Number | 6075050 |
| Brand | FastRNA™ |
| Components | RNApro™ Solution, Lysing Matrix E, Inhibitor Removal Solution, DEPC-treated H2O, RNAMATRIX® Binding Solution, RNAMATRIX® Slurry, RNAMATRIX® Wash Solution, Quick-Clean Spin Filters |
| FastPrep Instruments Models | ConeFlex Legacy (allows all FP24 adapters on FP96), CoolPrep (24x2 mL), HiPrep (48x2 mL), QuickFlex (96x2 mL), QuickPrep (24x2 mL) |
| Format | Kit |
| Pack Size | 50 Preps |
| Personal Protective Equipment | Personal-protective-equipment: If working with biological, toxic, hazardous or infectious samples; Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice. |
| Sample Source | Microorganisms |
| Sample Type | Feces, Soil, Wastewater, Environmental Water, Sludge |
| Sample Volume | up to 500 mg per prep |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [6075050] lot ID: 111330](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [6075050] lot ID: 111330")
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