经过独特的设计,FastDNA™ Spin土壤基因组试剂盒从土壤、淤泥等复杂环境样本中高效提取全部微生物的总DNA。多达500 mg的样本加入到裂解介质E管中,在FastPrepA™ -24 5G作用下,可以裂解多种疑难样本,例如真菌孢子、内生孢子、革兰氏阴性菌和酵母等,释放出来的DNA经过硅胶基质离心纯化,可直接用于PCR等下游实验。
- 产品优势
- 适用不同来源的土壤
- 无需有危害的有机试剂
- DNA产量高
- DNA纯度高
- 去除腐殖酸和PCR抑制剂
Application Notes
Fast DNA™ SPIN 土壤基因组DNA提取试剂盒用于从复杂环境样本(包括土壤、沉积物、污泥、堆肥、肥料、根际或废水)中高效提取全部微生物总DNA。包含裂解介质管和提取DNA所需的所有试剂,该过程可有效去除腐殖酸和其他PCR抑制剂。
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Used to isolate bacterial, fungal, plant and animal genomic DNA from soil and other environmental samples.
SKU | 116560900 |
Alternate Names | FastDNA Soil Kit |
Application Notes | Fast DNA™ SPIN 土壤基因组DNA提取试剂盒用于从复杂环境样本(包括土壤、沉积物、污泥、堆肥、肥料、根际或废水)中高效提取全部微生物总DNA。包含裂解介质管和提取DNA所需的所有试剂,该过程可有效去除腐殖酸和其他PCR抑制剂。 |
Base Catalog Number | 6560200 |
Brand | FastDNA™ |
Components | 50 x 2.0 mL tubes of Lysing Matrix E, 60 mL Sodium Phosphate Buffer, 8 mL MT Buffer, 25 mL of PPS Solution, 66 mL Binding Matrix, 50 Spin Modules, 50 Catch Tubes, 12 mL Concentrated SEWS-M, 20 mL DES, 200 µL BBS Gel Loading Dye |
FastPrep Instruments Models | ConeFlex Legacy (allows all FP24 adapters on FP96), CoolPrep (24x2 mL), HiPrep (48x2 mL), QuickFlex (96x2 mL), QuickPrep (24x2 mL) |
Format | Kit |
Hardness | Ceramic Spheres, Vickers 800; Silica Spheres, Knoop 515; Glass Bead, Knoop 515 |
Pack Size | 200 Preps |
Personal Protective Equipment | If working with biological, toxic, hazardous or infectious samples use hand protection (Latex, Nitrile or other protective approved material gloves), skin and body protection (Labcoat and long-sleeves), and eye protection (safety glasses with side-shields). Handle in accordance with good industrial hygiene and safety practices. |
pH | Reagent: SEWS-M pH Range: 6.4-6.8 |
Sample Type | Seeds, Spores, Bacteria (Gram +/-), Animal Tissues, Feces, Food, Fungi, Plants, Roots, Soil, Wastewater, Yeast |
Sample Volume | up to 500 mg per prep |
Specific Gravity | Ceramic Spheres, 4; Silica Spheres, 2.5; Glass Bead, 2.5 |
Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |