The FastDNA-96™ Fecal/Soil Microbe DNA Kit isolates microbial or host DNA from up to 80 mg of wet or dry stool or up to 135 mg of soil. Human DNA, gram (+/-) bacteria, fungi, parasites, algae, and spores found in fecal samples and microbial DNA found in different soil types (clay, sandy, silty, peaty, chalky, and loamy soils) are efficiently lysed in approximately 60 seconds with Lysing Matrix E (not included in kit) and the FastPrep-96™ Instrument. This kit efficiently removes humic acid and other PCR inhibitors commonly found in these sample types for fast, efficient, and inhibitor-free DNA extraction. Genomic DNA obtained from this kit is suitable for PCR, qPCR, NGS and other molecular downstream applications.
Application Notes
The FastDNA-96™ Fecal DNA Kit quickly and efficiently removes humic acid/polyphenols for inhibitor-free, PCR-ready genomic DNA extraction from stool (i.e. humans, primates, birds, rodents, cattle, canine, feline, porcine) and from a variety of soil samples including clay, sandy, silty, peaty, chalky, and loamy soils.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Used for Isolation of PCR-Ready Genomic DNA from Fecal Samples
| SKU | 119696400 |
| Application Notes | The FastDNA-96™ Fecal DNA Kit quickly and efficiently removes humic acid/polyphenols for inhibitor-free, PCR-ready genomic DNA extraction from stool (i.e. humans, primates, birds, rodents, cattle, canine, feline, porcine) and from a variety of soil samples including clay, sandy, silty, peaty, chalky, and loamy soils. |
| Base Catalog Number | 9696400 |
| Brand | FastDNA-96™ |
| Components | Components FastDNA-96™ Lysing Matrix Rack (0.5 mm Beads) 2 x 96-well rack, Lysis Buffer 2 x 40 mL, Fecal DNA Binding Solution 150 mL, Binding Plate Pre-Wash Buffer 50 mL, Fecal DNA Wash Buffer 100 mL, Elution Solution 2 x 10 mL, Inhibitor Plate Prep Solution 30 mL, Deep-Well Plate 2 each, MP-96 Binding Plate 2 each, MP-96 Inhibitor Removal Plate 2 each, Collection Plate 2 each, Elution Plate 6 each, Foil Plate Cover 4 each. |
| FastPrep Instruments Models | 2 x 96 Well Adapter |
| Format | Kit |
| Pack Size | 2 x 96 Preps |
| pH | pH Reagent: Lysis Buffer Lysis Buffer-pH: 8.0, Reagent: Fecal DNA Binding Solution Fecal DNA Binding Solution- pH Range: 8.5 - 9.5, Reagent: Binding Plate Pre-Wash Buffer Binding Plate Pre-Wash Buffer- pH Range: 7.0 - 8.0, Reagent: Fecal DNA Wash Buffer Fecal DNA Wash Buffer- pH Range: 7.5 - 8.0, Reagent: Elution Solution Elution Solution- pH Range: 7.0 - 8.0, Reagent: Inhibitor Plate Prep Solution Inhibitor Plate Prep Solution- pH Range: 6.0 - 7.0. |
| Sample Type | Soil, Mammalian Stool (i.e. humans, primates, birds, rodents, cattle, canine, feline, porcine, etc.) |
| Sample Volume | Up to 80 mg of Mammalian Stool (i.e. humans, primates, birds, rodents, cattle, canine, feline, porcine, etc.) or 135 mg of soil samples. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [9696400] lot ID: MP194609](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [9696400] lot ID: MP194609")
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