Rapid, High-Throughput Isolation of PCR-Ready Genomic DNA from Plant and Seed Samples The FastDNA™-96 Plant and Seed DNA Kit is designed for the simple and rapid isolation of PCR-quality DNA from vegatative samples in approximately 50 minutes. Plant samples including stems, roots, leaves, buds, flowers, fruits and seeds are efficiently lysed in approximately 60 seconds with the FastPrep-96™ Instrument. Up to 80 mg samples are placed into each 1.2 mL tube of a FastDNA-96™ Lysing Matrix Rack ( 96-deep well plate) containing 2.0 mm specially stabilized Zirconium oxide lysing particles, which are designed to efficiently lyse a wide variety of plant specimens, while in the presence of a specially formulated lysis solution. The cleared lysate is then applied to a binding matrix to isolate the DNA, and spin column plates are used to simplify liquid handling. Eluted DNA is then applied to the MP-96 Inhibitor Removal Plate which removes tannins, polyphenols and polysaccharides that can inhibit PCR. Purified DNA is eluted in an EDTA-free, DNA elution solution, and is ready for downstream applications including digestion, electrophoresis, arrays and PCR (A260/A280 ratios ≥ 1.8). Yield is typically 5 μg of total DNA eluted in 50-100 μL of elution solution. This procedure can be performed in minutes, and there is no need for organic denaturants or proteinases.
Application Notes
The FastDNA™-96 Plant/Seed DNA Kit for FastPrep® -96 is used for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, and seeds. Eluted DNA can be used for downstream molecular-based applications including PCR, arrays, digestion, electrophoresis etc.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
High-Throughput; Rapid Isolation of PCR-Ready Genomic DNA from Plant and Seed Samples using the FastPrep-96™ System
| SKU | 119696600 |
| Alternate Names | 96 well DNA purification kit |
| Application Notes | The FastDNA™-96 Plant/Seed DNA Kit for FastPrep® -96 is used for the simple, rapid isolation of inhibitor-free, PCR-quality DNA from a variety of plant sample sources including leaves, stems, buds, flowers, fruit, and seeds. Eluted DNA can be used for downstream molecular-based applications including PCR, arrays, digestion, electrophoresis etc. |
| Base Catalog Number | 9696600 |
| Brand | FastDNA™ |
| Components | FastDNA-96&trade||Lysing Matrix Rack (2.0 mm Beads) 2 x 96-well rack||Lysis Buffer 2 x 40 mL||Plant/Seed DNA Binding Solution 150 mL||Binding Plate Pre-Wash Buffer 50 mL||Plant/Seed DNA Wash Buffer 100 mL||Elution Solution 2 x 10 mL||Inhibitor Plate Prep Solution |
| FastPrep Instruments Models | 2 x 96 Well Adapter |
| Format | Kit |
| Hardness | Mohs 9, Rockwell A >87, Vickers >1350 |
| Pack Size | 2 x 96 Preps |
| Personal Protective Equipment | Personal-protective-equipment: If working with biological, toxic, hazardous or infectious samples; Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice. |
| Sample Source | Plant Tissues |
| Sample Type | Seeds, Plants, Roots, Buds, Flowers, Fruits, Leaves, Stems |
| Sample Volume | up to 80 mg |
| Specific Gravity | 6.02 |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [9696600] lot ID: QR15513](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [9696600] lot ID: QR15513")
