3-(4,5-Dimethylthiazolyl-2)-2,5-diphenyl tetrazolium bromide, cell culture reagent

Name: 3-(4,5-Dimethylthiazolyl-2)-2,5-diphenyl tetrazolium bromide, cell culture reagent
SKU: 02194592-CF

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Key features and details

3-(4,5-Dimethylthiazolyl-2)-2,5-Diphenyl Tetrazolium Bromide

SKU: 02194592-CF

Synonyms

MTT; Thiazolyl Blue;3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide;Methylthiazolyldiphenyl-tetrazolium bromide

CAS Number:

298-93-1

Molecular Formula:

C18H16BrN5S

Molecular Weight:

414.325 g/mol

Beilstein Registry Number:

4081397

EC Number:

206-069-5

MDL Number:

MFCD00011964

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Product Description

3-(4,5-Dimethylthiazolyl-2)-2,5-Diphenyl Tetrazolium Bromide

Application Notes

3-(4,5-Dimethyl-2-thiazolyl-2)-2,5-diphenyl-tetrazolium bromide is used as a colorimetric metabolic activity indicator in cell viability assays. Thiazolyl Blue Tetrazolium Blue (MTT) is a dye used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Colorimetric assay; Cell viability; cell culture

Specifications

SKU	02194592-CF
Alternate Names	MTT; Thiazolyl Blue;3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide;Methylthiazolyldiphenyl-tetrazolium bromide
Application Notes	3-(4,5-Dimethyl-2-thiazolyl-2)-2,5-diphenyl-tetrazolium bromide is used as a colorimetric metabolic activity indicator in cell viability assays. Thiazolyl Blue Tetrazolium Blue (MTT) is a dye used in measurement of cell proliferation. MTT produces a yellowish solution that is converted to dark blue, water-insoluble MTT formazan by mitochondrial dehydrogenases of living cells. The blue crystals are solubilized with acidified isopropanol and the intensity is measured colorimetrically at 570 nm. MTT has been used as a histochemical/cytochemical reagent and for the detection of NAD. ADP-linked enzyme systems in tissue cannot be detected with MTT, due to binding of the cation by the cyanide trap used. MTT is rapidly reduced to the formazan, which chelates with nickel, copper, and cobalt; the cobalt chelate has been used in oxidative systems.
Base Catalog Number	194592
Beilstein Registry Number	4081397
CAS #	298-93-1
EC Number	206-069-5
Grade	Cell Culture Grade
Hazard Statements	H315-H319-H335-H341
Melting Point	195 deg C (decomposes)
Molecular Formula	C18H16BrN5S
Molecular Weight	414.325 g/mol
Personal Protective Equipment	Eyeshields, Gloves, respirator filter
Purity	~98%
RTECS Number	XF8060000
Safety Symbol	GHS07, GHS08
Solubility	Solubility in <a class="pubchem-internal-link CID-8019" href="https://pubchem.ncbi.nlm.nih.gov/compound/2-methoxyethanol">2-methoxyethanol</a>, 20 mg/mL; <a class="pubchem-internal-link CID-702" href="https://pubchem.ncbi.nlm.nih.gov/compound/ethanol">ethanol</a>, 20 mg/mL
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
UV Visible Absorbance	376 ± 2 nm; 242 ± 2 nm λmax (methanol)
Vapor Pressure	5.11X10-11 mm Hg at 25 deg C /Estimated/