Bis-Tris
Application Notes
Bis-Tris is suitable for fractionation of hemoglobin. A protocol for the agarose gel electrophoresis of glyoxylated RNA that uses Bis-Tris in the electrophoresis buffer has been described. The use of bis-Tris in polyacrylamide gels for the in-gel derivatization of proteins for cysteine-specific cleavages and MS analysis has been reported. A multiphasic buffer system for the SDS-PAGE analysis of proteins and peptides in the molecular weight range of 1-100 kDa that incorporates bis-Tris as a counterion in the stacking phase has been described. Bis-Tris buffer has been utilized in a study of λ cro repressor self-assembly and dimerization.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Used in electrophoresis buffer
SKU | 02194546-CF |
Alternate Names | 2,2-Bis-(hydroxymethyl)-2,2´,2´´- nitrilotriethanol; bis(2-hydroxymethyl)imino-tris (hydroxymethyl)methane; 2-bis(2-Hydroxyethyl)amino-2-(hydroxymethyl)-1,3-propandiol |
Application Notes | Bis-Tris is suitable for fractionation of hemoglobin. A protocol for the agarose gel electrophoresis of glyoxylated RNA that uses Bis-Tris in the electrophoresis buffer has been described. The use of bis-Tris in polyacrylamide gels for the in-gel derivatization of proteins for cysteine-specific cleavages and MS analysis has been reported. A multiphasic buffer system for the SDS-PAGE analysis of proteins and peptides in the molecular weight range of 1-100 kDa that incorporates bis-Tris as a counterion in the stacking phase has been described. Bis-Tris buffer has been utilized in a study of λ cro repressor self-assembly and dimerization. |
Base Catalog Number | 194546 |
Beilstein Registry Number | 2205275 |
CAS # | 6976-37-0 |
EC Number | 230-237-7 |
Extinction Coefficient | EM (water): 259 nm = 10; 277 nm = 10 (Lit.) |
Format | Powder |
Grade | Cell Culture Grade |
Hazard Statements | H315-H319-H335 |
Molecular Formula | C8H19NO5 |
Molecular Weight | 209.242 g/mol |
Personal Protective Equipment | Dust mask, Eyeshields, Gloves |
pKa | 6.5 (25 °C)(Lit.) |
Purity | ≥99% |
Safety Symbol | GHS07 |
Sterilization of Solutions | For sterilization of aqueous solutions, MP Biomedicals recommends sterile filtration with a 0.2 µm filter; however, the product is typically stable for autoclaving. |
Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |