Brefeldin A
Application Notes
Brefeldin A reversibly inhibits the intracellular translocation of proteins in eukaryotes, e.g., during transport of proteins to the cell surface for secretion or expression. It has been reported to block the response of cultured cells to cholera toxin. BFA inhibits protein synthesis in cultured cells and inhibits the transport of secretory and lysosomal proteins at concentrations of 1-10 µg/mL. In HepG2 cells, BFA induces two blocks in the secretory pathway; one at the level of the endoplasmic reticulum-Golgi juncture and the other in the trans-Golgi network. Brefeldin A is used in the studies of Brefeldin A-inhibited Guanine Nucleotide-exchange Protein, BIG2, Regulates the Constitutive Release of TNFR1 Exosome-like Vesicles.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Brefeldin A blocks protein transportation into post-Golgi compartments.
| SKU | 02159027-CF |
| Alternate Names | (-4-Dihydroxy-2-[6-Hydroxy-1-Heptenyl]-4-Cyclopentane Crotonic Acid 8-Lactone; BFA; Ascotoxin; Cyanein; Decumbin |
| Application Notes | Brefeldin A reversibly inhibits the intracellular translocation of proteins in eukaryotes, e.g., during transport of proteins to the cell surface for secretion or expression. It has been reported to block the response of cultured cells to cholera toxin. BFA inhibits protein synthesis in cultured cells and inhibits the transport of secretory and lysosomal proteins at concentrations of 1-10 µg/mL. In HepG2 cells, BFA induces two blocks in the secretory pathway; one at the level of the endoplasmic reticulum-Golgi juncture and the other in the trans-Golgi network. Brefeldin A is used in the studies of Brefeldin A-inhibited Guanine Nucleotide-exchange Protein, BIG2, Regulates the Constitutive Release of TNFR1 Exosome-like Vesicles. |
| Base Catalog Number | 159027 |
| Beilstein Registry Number | 25191 |
| Biochemical Physiological Actions | Brefeldin A (BFA) is a fungal metabolite which disrupts the structure and function of the Golgi apparatus. BFA is an activator of the sphingomyelin cycle. Brefeldin A-mediated apoptosis has been observed in human tumor cells. |
| CAS # | 20350-15-6 |
| Hazard Statements | H301 |
| Molecular Formula | C16H24O4 |
| Molecular Weight | 280.364 g/mol |
| Personal Protective Equipment | Dust mask, Eyeshields, Faceshields, Gloves |
| Purity | ≥98% |
| RTECS Number | GY8410000 |
| Safety Symbol | GHS06 |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [159027] lot ID: 1172H](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [159027] lot ID: 1172H")
Powered by Bioz