The anti-globulin or Coombs' test is widely used to detect the presence of antibody in the diagnosis of autoimmune hemolytic anemia. Antibody alone or in the presence of complement injures the cell membrane permitting leakage of RBC material, allowing recognition by the reticuloendothelial cells, thereby leading to phagocytosis and destruction of RBC's. Anti-globulin reagent (Coombs' serum) is prepared by immunizing rabbits with canine IgG, IgM and C3 purified from normal serum. The resulting whole antiserum is heat treated to inactivate complement and is adsorbed with normal pooled canine erythrocytes to remove nonspecific agglutinins.
Application Notes
Canine anti-globulin or Coombs' test is widely used to detect the presence of antibody in the diagnosis of autoimmune hemolytic anemia. Antibody alone or in the presence of complement injures the cell membrane permitting leakage of RBC material, allowing recognition by the reticuloendothelial cells, thereby leading to phagocytosis and destruction of RBC's. Immune mediated erythrocyte destruction is also seen in canine systemic lupus erythematosus some infections and in neoplastic disorders.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Immunoassays | Autoimmune Hemolytic Anemia (AIHA) diagnosis
| SKU | 0864635-CF |
| Antibody Type | Polyclonal Antibody |
| Application Notes | Canine anti-globulin or Coombs' test is widely used to detect the presence of antibody in the diagnosis of autoimmune hemolytic anemia. Antibody alone or in the presence of complement injures the cell membrane permitting leakage of RBC material, allowing recognition by the reticuloendothelial cells, thereby leading to phagocytosis and destruction of RBC's. Immune mediated erythrocyte destruction is also seen in canine systemic lupus erythematosus some infections and in neoplastic disorders. |
| Base Catalog Number | 646351 |
| Conjugate | Unconjugated |
| Disease Screened | Coombs' test |
| Format | lyophilized |
| Formulation Details | Lyophilized |
| Grade | Molecular Grade |
| Host | Rabbit |
| Preparation Method | Anti-globulin reagent (Coombs' serum) is prepared by immunizing rabbits with canine IgG, IgM and C3 purified from normal serum. The resulting whole antiserum is heat treated to inactivate complement and is adsorbed with normal pooled canine erythrocytes to remove nonspecific agglutinins. Canine anti-globulin reagent is presented in lyophilized form. One mL of reconstituted material is sufficient for 10 tests by the tube method or 50 when using the microtiter plate method. |
| Solubility | Soluble in water |
| Species Reactivity | Dog |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [646351] lot ID: 08241](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [646351] lot ID: 08241")