The RNaid™ Kit contains all of the solutions and reagents, except for ethanol, necessary for the isolation and purification of RNA in twenty minutes from agarose and polyacrylamide gels, from solutions, and from unincorporated radioactive label. The resulting RNA is suitable as a substrate for multiple enzymatic manipulations including reverse transcription, RNase protection assays, and in vitro translation.
Application Notes
The RNaid™ Kit is designed to isolate and purify RNA (75 bases to 10 kb) from native and denaturing agarose and polyacrylamide gels. Removal of unincorporated nucleotides, buffer salts, and other reagents from enzyme reactions. The purified RNA is ready for reverse transcription and PCR, labeling and microarray analysis, and other common applications.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Isolate and purify RNA from native and denaturing agarose and polyacrylamide gels and from solutions
SKU | 111007200 |
Alternate Names | RNA Clean-Up Kit |
Application Notes | The RNaid™ Kit is designed to isolate and purify RNA (75 bases to 10 kb) from native and denaturing agarose and polyacrylamide gels. Removal of unincorporated nucleotides, buffer salts, and other reagents from enzyme reactions. The purified RNA is ready for reverse transcription and PCR, labeling and microarray analysis, and other common applications. |
Base Catalog Number | 1007200 |
Brand | RNaid™ |
Components | DEPC-Treated Water| RNA Binding Salt| RNA Wash Concentrate| RNAMATRIX™| 10% Acetic Acid |
Format | RNAMATRIX™ slurry |
Pack Size | 200 Preps |
Personal Protective Equipment | Personal-protective-equipment: Hand protection: Latex, Nitrile or other plastic material gloves. Skin and body protection: Labcoat and sleves. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice. |
Preparation Method | RNA Wash Concentrate needs to be prepared before use in the protocol. Add 120 ml of 100% Ethanol to the bottle of RNA Wash Concentrate and mix well before use. Write preparation date on the label. Keep tightly capped to prevent EtOH evaporation. |
Recommended Alternate | RNaid™ SPIN Kit |
Sample Source | Gels and Solutions |
Sample Type | Enzymatic Reactions, Solutions, Agarose Gels, Polyacrylamide Gels |
Sample Volume | Up to ~0.5 ml gel slice by volume or ~1.0 ml solution |
Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |