Pure Canavalia ensiformis lectin (Con A) from Jackbean. Isolated by affinity chromatography on cross-linked dextran. Con A exists as a dimer below pH 5.0 and and at pH >7 it exists as a tetramer. Con-A is not a glycoprotein. The monomeric molecular weight of Con-A is 25,500. Con-A does not contain cysteine residues. Unlike most other lectins, Con-A is a metalloprotein and requires a transition metal ion, such as manganese, plus calcium ions for binding. Lectins are proteins or glycoproteins of non-immune origin that agglutinate cells and/or precipitate complex carbohydrates. Lectins are capable of binding glycoproteins even in presence of various detergents. The agglutination activity of these highly specific carbohydrate-binding molecules is usually inhibited by a simple monosaccharide, but for some lectins, di, tri, and even polysaccharides are required.
Application Notes
Lectin are used in a wide variety of applications in vitro, including: blood grouping and erythrocyte polyagglutination studies, Mitogenic stimulation of lymphocytes, Lymphocyte subpopulation studies, Fractionation of cells and other particles, Histochemical studies of normal and pathological conditions. Con-A has been used to elucidate structural changes in the membrane surface of transformed cells. Con-A conjugates of antitumor drugs have been used in drug delivery systems in cultured cells.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Cell Biology | Cell Signaling
| SKU | 02195283-CF |
| Alternate Names | Con A |
| Application Notes | Lectin are used in a wide variety of applications in vitro, including: blood grouping and erythrocyte polyagglutination studies, Mitogenic stimulation of lymphocytes, Lymphocyte subpopulation studies, Fractionation of cells and other particles, Histochemical studies of normal and pathological conditions. Con-A has been used to elucidate structural changes in the membrane surface of transformed cells. Con-A conjugates of antitumor drugs have been used in drug delivery systems in cultured cells. |
| Base Catalog Number | 195283 |
| Biochemical Physiological Actions | Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6. Con-A binds specifically to mannosyl and glucosyl residues of polysaccharides and glycoproteins. Unmodified hydroxyl groups at the C3, C4 and C6 positions of D-glucopyranosyl or D-mannopyranosyl rings may be essential for binding. Each subunit of Con-A contains one calcium ion and one manganese ion. Removal of these cations by dialysis under acidic conditions abolishes the carbohydrate-binding activity. |
| CAS # | 11028-71-0 |
| EC Number | 234-258-2 |
| Extinction Coefficient | 280nm = 11.4 (0.1 M NaCl)(Lit.) |
| Format | Powder, Essentially Salt Free, Highly Purified |
| Grade | Higly Purified |
| Hazard Statements | H317-H334-H361 |
| Isoelectric Point | 4.5, 4.7, 5.0-5.1, and 5.4-5.5 (several isoforms)(Lit.) |
| Personal Protective Equipment | Dust mask, Eyeshields, Faceshields, Gloves |
| RTECS Number | GK6890000 |
| Safety Symbol | GHS08 |
| Solubility | Soluble in water (10 mg/ mL-slightly hazy, colorless solution)(40 mg/mL in 0.01 M PBS, pH 6.8 containing 0.1 mM Ca2+, 0.1 mM Mn2+). |
| Source | Canavalia ensiformis seeds (jack beans) |
| Specificity | Carbohydrate Specificity: a-Mannose; a-Glucose; Branched Mannose. |
| Typical Working Concentration | Reconstitution: Reconstitute with buffer to a final concentration of 1 mg/mL. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [195283] lot ID: 1343J](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [195283] lot ID: 1343J")
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