Dosage of DSS for different strains of mice |
Animal/Strain |
Dose |
Days |
Publication |
C57BL/6 |
2.5% |
8 |
Jia, Q.; Ivanov, I.; Zlatev, Z.; et al. Dietary fish oil and curcumin combine to modulate colonic cytokinetics and gene expression in dextran sodium sulphate-treated mice. Br.J.Nutr. 2011, 106(4), 519-9. |
Wild-type C57BL/6J(m) |
3% |
6 |
Thiess, A.L.; Laroui, H.; Obertone, T.S.; et al. Nanoparticle-based therapeutic delivery of prohibitin to the colonic epithelial cells ameliorates acute murine colitis. Inflamm. Bowel Dis. 2011, 17(5), 1163-76. |
C57BL/6 AhR null, WT |
3.5% |
7 |
Arsenescu, R.; Arsenescu, V.; Zhong, J.; et al. Role of xenobiotic receptor in inflammatory bowel disease. Inflamm. Bowel Dis. 2011, 17(5), 1149-2. |
C57BL/6 |
5% |
3-14 |
Nagalingham, N.A.; Kao, J.Y.; Young, V.B. Microbial ecology of the murine gut associated with the development of dextran sodium sulfate-induced colitis. Inflamm, Bowel Disease. 2011, 7(4), 917-26. |
C57BL/6 |
1.5% |
7 |
Ramakers, J.; Verstege, M.I.; Thuijls, G.; et al. The PPARγ agonist rosiglitazone impairs colonic inflammation in mice with experimental colitis. J.Clin.Immunol. 2007, 27(3), 275-283. |
BALB/c |
1% |
10 |
Palffy, R.; Gardlik, R.; Behuliak, M.; et al. Salmonella-mediated gene therapy in experimental colitis in mice. Ex.Biol.Med. 2011, 236(2), 177-83. |
C57BL/6J |
3% |
5 |
Shiomi, Y.; Nishiumi, S.; Ooi, M.; et al. GCMS-based metabolomic study in mice with colitis induced by dextran sulfate sodium. Inflamm. Bowel Dis. 2011, 17(11), 2261-74. |
BALB/c |
1-5% |
10 |
Rochat, T.; Bermudez-Humaran, L.; Gratadoux, J-J.; et al. Anti-inflammatory effects of Lactobacillus casei BL23 producing or not a manganese-dependent catalase on DSSinduced colitis in mice. Microb. CellFact. 2007, 20(6), 22. |
BALB/c; NMRI/KI |
2.5-5% |
n/a |
Bylund-Fellenius, A-C.; Landström, E.; Axelsson, L.G.; et al. Experimental colitis induced by dextran sulphate in normal and germfree mice. Microbial Ecology in Health and Disease. 1994, 7, 207-215. |
IL-5-/- and +/+ |
2.9%, 5% |
9 |
Stevceva, L.; Pavli, P.; Husband, A.; et al. Eosinophilia is attenuated in experimental colitis induced in IL-5 deficient mice. Genes Immun. 2000, 1(3), 213-8 |
BALB/c; athymic nu/nu CD-1 (BR) |
2.5-5% |
7-35 |
Axelsson, L.G.; Landström, E.; Bylund-Fellenius, A.C. Experimental colitis induced by dextran sulphate sodium in mice: Beneficial effects of sulphasalazine and olsalazine. Aliment. Pharmacol.Ther. 1998, 12(9), 925-34. |
WT; CCR9(-/-); CCL25 (-/-) |
2% |
7 |
Wurbel, M.A.; McIntyre, M.G.; Dwyer, P.; et al. CCL25/CCR9 interactions regulate large intestinal inflammation in a murine model of acute colitis. PLoS One. 2011, 6(1), e16442. |
Wild-type; DPIV -/- |
2% |
6 |
Yazbeck, R.; Howard, G.S.; Butler, R.N.; et al. Biochemical and histological changes in the small intestine of mice with dextran sulfate sodium induced colitis. J.Cell Physiol. 2011, 226(12), 319-24. |
BALB/c |
5% |
7 |
Kumar, G.K.; Dhamotharan, R.; Kulkarni, N.M. Embelin ameliorates dextran sodium sulfate-induced colitis in mice. Int. Immunopharmacol. 2011, E |
Dosage of DSS for different strains of rats |
Animal/Strain |
Dose |
Days |
Publication |
Wistar |
2% |
2 weeks to 6 months |
Tamaru, T.; Kobayashi, H.; Kishimoto, S.; et al. Histochemical study of colonic cancer in experimental colitis of rats. Dig. Dis. Sci. 1993, 38, 529-537. |
Sprague-Dawley |
5% |
9 |
Schreiber, O.; Petersson, J.; Phillipson, M.; et al. Lactobacillus reuteri prevents colitis by reducing P-selectin associated leukocyte- and platelet-endothelial cell interactions.
Am.J.Physiol.Gastrointest.Liver. 2009, 296, G534-542. Dicksved, J.; Schreiber, O.; Willing, B.; et al. Lactobacillus reuteri maintains a functional mucosal barrier during DSS treatment despite mucus layer dysfunction. PLoS One. 2012, 7(9), e46399.
Petersson, J.; Schreiber, O.; Steege, A.; et al. eNOS involved in colitis-induced mucosal blood flow increase. Am.J.Physiol.Gastrointest.Liver. 2007, 293, G1281-1287.
|
Sprague-Dawley |
5% |
6 |
Vasina, V.; Broccoli, M.; Ursino, M.G.; et al. Non-peptidyl low molecular weight radical scavenger IAC attenuates DSS-induced colitis in rats. World J.Gastroenterol. 2010, 16(29), 3642-50. |
Sprague-Dawley |
5% |
7 |
Shi, X.Z.; Winston, J.H.; Sarna, S.K. Differential immune and genetic responses in rat models of Crohn’s colitis and ulcerative colitis. Am.J.Physiol.Gastrointest.Liver Physiol. 2011, 300(1), G41-51. |
Wistar |
2.5% |
7 |
Aoi, Y.; Terashima, S.; Ogura M.; et al. Roles of nitric oxide (NO) and NO synthases in healing of dextran sulfate sodium-induced rat colitis. J Physio Pharmacol. 2008, 59(2), 315-36. |
Wistar |
5% |
10 |
Lopez-Posadeas, R.; Requena, P.; Gonzalez, R.; et al. Bovine glycomacropeptide has intestinal antiinflammatory effects in rats with dextran-sulfate induced colitis. J.Nutr. 2010, 140(11), 2014-2019. |
Wistar |
2-4% |
7 |
Shimizu, T.; Suzuki, M.; Fujimura, J.; et al. The relationship between the concentration of dextran sodium sulfate and the degree of induced experimental colitis in weanling rats. J.Pediatric Gastro. Nutrition. 2003, 37, 481-486. |
ACI |
5% |
14 |
Hirono, I.; Kuhara, K.; Hosaka, S.; et al. Induction of intestinal tumors in rats by dextran sulfate sodium. J.Natl.Cancer Inst. 1981, 66(3), 579-583. |
Dosage of DSS for other animals |
Animal/Strain |
Dose |
Days |
Publication |
Hamster |
2.5% |
6 |
Karlsson, A.; Jägervall, A.; Pettersson, M.; et al. Dextran sulphate sodium induces acute colitis and alters hepatic function in hamsters. Int. Immunopharmacol. 2008, 8(1), 20-27 |
Hamster |
1% |
N/A |
Yamada, M.; Ohkusa, T.; Ohkusa, I. Occurence of dysplasia and adenocarcinoma after experimental chronic ulcerative colitis in hamsters induced by dextran sulphate sodium. Gut. 1992, 33, 1521-1527 |
Guinea Pig |
3% |
4 |
Iwanaga, T.; Hoshi, O.; Han, H.; et al. Morphological analysis of acute ulcerative colitis experimentally induced by dextran sulfate sodium in the guinea pig: Some possible mechanisms of cecal ulceration. J. Gastroenterol. 1994, 29(4), 430-438. |
Pig (Yorkshire) |
1.25 g/kg BW |
5 |
Young, D.; Ibuki, M.; Nakamori, T.; et al. Soy-derived di-and tripeptides alleviate colon and ileum inflammation in pigs with dextran sodium sulfate-induced colitis. J.Nutr. 2012, 142(2), 363-8. |