Ethylenediaminetetraacetic acid, disodium salt, dihydrate, cell culture reagent, ≥99%, 100 g
Ethylenediamine Tetraacetic Acid is a polyamino carboxylic acid hexadentate ligand.
Application Notes
Ethylenediamine tetraacetic acid (EDTA) is a calcium chelator used to eliminate inhibition of enzyme catalyzed reactions due to traces of heavy metals. For use as an anticoagulant, disodium or tripotassium salts of EDTA are most commonly used. EDTA prevents platelet aggregation and is therefore the preferred anticoagulant for platelet counts. It is an inhibitor of metalloproteases and metal-activated proteases. It acts as a chelator of the zinc ion in the active site of metalloproteases, but EDTA can also inhibit other metal ion-dependent proteases such as calcium-dependent cysteine proteases. EDTA interferes with biological processes which are metal-dependent. It is a buffer component.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Chelating agent
| SKU | 0219465980 |
| Alternate Names | EDTA; (Ethylenedinitrilo)tetraacetic acid; Edathamil; Ethylenedinitrilotetraacetic acid |
| Application Notes | Ethylenediamine tetraacetic acid (EDTA) is a calcium chelator used to eliminate inhibition of enzyme catalyzed reactions due to traces of heavy metals. For use as an anticoagulant, disodium or tripotassium salts of EDTA are most commonly used. EDTA prevents platelet aggregation and is therefore the preferred anticoagulant for platelet counts. It is an inhibitor of metalloproteases and metal-activated proteases. It acts as a chelator of the zinc ion in the active site of metalloproteases, but EDTA can also inhibit other metal ion-dependent proteases such as calcium-dependent cysteine proteases. EDTA interferes with biological processes which are metal-dependent. It is a buffer component. |
| Auto Ignition | > 200 °C (DIN 51794) (Lit.) |
| Base Catalog Number | 19465980 |
| Beilstein Registry Number | 1716295 |
| CAS # | 60-00-4 |
| Density | ca. 860 kg/m<sup>3</sup> (bulk density) (Lit.) |
| EC Number | 200-449-4 |
| Flash Point | > 100 °C (closed cup and DIN 51758) (Lit.) |
| Grade | Cell Culture Grade |
| Hazard Statements | H319 |
| Melting Point | 250 °C (dec.), 236 - 246 °C (Lit.) |
| Molecular Formula | C10H16N2O8 |
| Molecular Weight | 292.3 |
| Pack Size | 100 g |
| Personal Protective Equipment | Dust mask, Eyeshields, Gloves |
| pH | 2 - 3 (saturated solution) |
| pKa | 2.0, 2.7, 6.2, 10.3 (Lit.) |
| Purity | ≥99% |
| RTECS Number | AH4025000 |
| Safety Symbol | GHS07 |
| Solubility | Soluble in 3M NaOH (160 mg/mL); 1 M aq soln: Clear, Colorless & Complete, No Black Specks, Haze Free. |
| Typical Working Concentration | EDTA is an inhibitor of metalloproteases and metal-activated proteases at an effective concentration of 1-10 mM. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |