Glycine
Application Notes
Glycine is widely used as a buffer for a variety of immunological applications. Glycine is also used as an eluent in column chromatography. Glycine has been reported for use in the enzymatic determination of lactate using lactic dehydrogenase. Commonly used as a component in Tris-glycine and Tris-glycine-SDS running buffers for polyacrylamide gel electrophoresis, a component of Towbin's transfer buffer for Western blots , a buffer substance in cryoenzymology, in osmotic pressure maintenance in isoelectric focusing of erythrocytes, salting-in effect in protein chemistry, and as a buffer component in the coupled phosphatase-kinase reaction for end labelling of restriction fragments.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
PAGE compound
| SKU | 02194049-CF |
| Alternate Names | Aminoacetic acid; Glycolixir; Hampshire Glycine; Aminoethanoic acid; Glycocoll; G; Gly |
| Application Notes | Glycine is widely used as a buffer for a variety of immunological applications. Glycine is also used as an eluent in column chromatography. Glycine has been reported for use in the enzymatic determination of lactate using lactic dehydrogenase. Commonly used as a component in Tris-glycine and Tris-glycine-SDS running buffers for polyacrylamide gel electrophoresis, a component of Towbin's transfer buffer for Western blots , a buffer substance in cryoenzymology, in osmotic pressure maintenance in isoelectric focusing of erythrocytes, salting-in effect in protein chemistry, and as a buffer component in the coupled phosphatase-kinase reaction for end labelling of restriction fragments. |
| Base Catalog Number | 194049 |
| Beilstein Registry Number | 635782 |
| Biochemical Physiological Actions | Inhibitory neurotransmitter in spinal cord, allosteric regulator of NMDA receptors. |
| CAS # | 56-40-6 |
| Density | 1.1607 (NTP, 1992) |
| EC Number | 200-272-2 |
| Format | Crystalline powder |
| Grade | Reagent Grade |
| Melting Point | 451° F (NTP, 1992) |
| Molecular Formula | C2H5NO2 |
| Molecular Weight | 75.067 g/mol |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| pH | 0.2 molar soln in <a class="pubchem-internal-link CID-962" href="https://pubchem.ncbi.nlm.nih.gov/compound/water">water</a>: 4.0 |
| pKa | 2.35 at 25 °C (Lit.) |
| RTECS Number | MB7600000 |
| Solubility | greater than or equal to 100 mg/mL at 64° F (NTP, 1992) |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [194049] lot ID: 5159A](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [194049] lot ID: 5159A")