异丙基-β-D-硫代半乳糖苷(IPTG), 分子生物学试剂, ,Isopropyl-β-D-thiogalactopyranoside, molecular biology, ≥99%
Isopropyl-Β-D-Thiogalactopyranoside
Application Notes
Isopropyl b-D-1-thiogalactopyranoside is a commonly used reagent in cloning procedures that require induction of b-galactosidase activity and is used in conjunction with X-Gal. It is commonly used in cloning procedures that require induction of β-galactosidase activity. It is also used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
β-Galactoside inducer; protein expression
| SKU | 02194029-CF |
| Alternate Names | IPTG; Isopropyl β-D-thiogalactoside; l-Propyl-b-D-thiogalactopyranoside |
| Application Notes | Isopropyl b-D-1-thiogalactopyranoside is a commonly used reagent in cloning procedures that require induction of b-galactosidase activity and is used in conjunction with X-Gal. It is commonly used in cloning procedures that require induction of β-galactosidase activity. It is also used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ. |
| Base Catalog Number | 194029 |
| Beilstein Registry Number | 4631 |
| CAS # | 367-93-1 |
| EC Number | 206-703-0 |
| Grade | Molecular Biology Grade |
| Hazard Statements | H319-H351 |
| Melting Point | 121 °C |
| Molecular Formula | C9H18O5S |
| Molecular Weight | 238.298 g/mol |
| Optical Rotation | (c=1, water)-26 ° to -33 ° |
| Purity | ≥99% |
| Safety Symbol | GHS07,GHS08 |
| Sterilization of Solutions | Solutions can be sterile filtered using a 0.2 mm filter. |
| Typical Working Concentration | The final concentration typically used in indicator plates is 0.2 mM. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [02194029-CF] lot ID: YC11035](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [02194029-CF] lot ID: YC11035")