Peptide thiobenzyl esters are very sensitive substrates for serine and cystein proteases. The thiol leaving group is detected by reaction with a thiol reagent such as 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB or Leman's reagent) or 4,4'-dithiodipyridine. The rate of thiobenzyl ester hydrolysis is then measured at 324 nm by monitoring the formation of 4-thiopyridone (E324nm = 19,800 M-1cm-1) or at 405 nm using DTNB as a thiol reagent (E405nm = 13,260 M-1cm-1) for 2-nitro-5-thiobenzoate anion TNBS. A chromogenic substrate for Plasmogen Activator Serine Protease
Application Notes
Research use or further manufacturing
Key Applications
A chromogenic substrate for Plasmogen Activator Serine Protease.
SKU | 03SB007-CF |
Alternate Names | Z-Gly-Arg-SBzl, Z-GR-Thiobenzyl Ester |
Application Notes | Research use or further manufacturing |
Format | Semisolid |
Formulation Details | C23H29N5O4S |
Molecular Formula | C23H29N5O4S |
Molecular Weight | 471.57 (Not including TFA salt) |
Protein or Enzyme Type | Activators, Inhibitors & Substrates |
Purity | ≥95% |
Solubility | Soluble in DMSO (Dimethyl Sulfoxide) and water. |
Storage | Keep cool and dry; Hygroscopic, store in freezer. |