Bilirubin Oxidase
Application Notes
Bilirubin oxidase is used to degrade bilirubin. Bilirubin oxidase, from Myrothecium verrucaria, may be used to determine free hemoglobin in icteric specimens. It also has potential application in dye effluent decolorization and is a potential treatment for neonatal jaundice. It is used in diagnostic determination to determine two types of bilirubin, glucuronide-conjugated and unconjugated bilirubin separately. Also in various enzymatic assay using oxidase-peroxidase such as "Glucose oxidase system", "Cholesterol oxidase system", "Acyl coenzyme A oxidase system", and "Glycerol-3-phosphate oxidase system", the accuracy of the assay system can be increased as the interference of bilirubin is removed by oxidation of bilirubin in body fluid with bilirubin oxidase.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
| SKU | 02190000-CF |
| Alternate Names | Bilirubin:oxygen oxidoreductase |
| Application Notes | Bilirubin oxidase is used to degrade bilirubin. Bilirubin oxidase, from Myrothecium verrucaria, may be used to determine free hemoglobin in icteric specimens. It also has potential application in dye effluent decolorization and is a potential treatment for neonatal jaundice. It is used in diagnostic determination to determine two types of bilirubin, glucuronide-conjugated and unconjugated bilirubin separately. Also in various enzymatic assay using oxidase-peroxidase such as "Glucose oxidase system", "Cholesterol oxidase system", "Acyl coenzyme A oxidase system", and "Glycerol-3-phosphate oxidase system", the accuracy of the assay system can be increased as the interference of bilirubin is removed by oxidation of bilirubin in body fluid with bilirubin oxidase. |
| Base Catalog Number | 190000 |
| Biochemical Physiological Actions | Bilirubin oxidase (BOD) is a multicopper oxidase that catalyzes the oxidation of bilirubin to biliverdin. Oxygen is the electron acceptor and is reduced to water. The enzyme is used for diagnostic analysis of bilirubin in serum during medical examinations of the serum. It has also been used as an enzymatic catalyst for the cathode of biofuel cells that work under neutral conditions. It is a monomeric protein with a molecular mass of 60 kDa and high reactivity at neutral pH. BOD participates in porphyrin and chlorophyll metabolism. |
| CAS # | 80619-01-8 |
| Format | Powder |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| Source | Myrothecium species |
| Specific Activity | ≥0.8 u/mg solid |
| Unit Definition | One unit is the amount of enzyme that oxidizes one micromole of bilirubin per minute at 37 °C, pH 8.4. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [190000] lot ID: 3168J](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [190000] lot ID: 3168J")