凝血酶广泛应用于诊断行业中,用于凝血实验,凝血因子测试以及全血或血浆的去纤维化以用于血清的对照实验。凝血酶还可用于重组融合蛋白的特异性位点酶切等生化实验中。
低比活性的牛凝血酶以新西兰源牛血浆为原料制备凝血酶原,使用从新西兰源的牛肺组织中提取的凝血活酶激活凝血酶原,通过分离纯化获得,并在真空下冷冻干燥以粉末的形式供应。低比活力凝血酶的比活力为90-300 NIH U/mg蛋白,总蛋白含量大于50%。
Application Notes
凝血酶广泛应用于诊断行业中,用于凝血实验,凝血因子测试以及全血或血浆的去纤维化以用于血清的对照实验。凝血酶还可用于重组融合蛋白的特异性位点切割等生化实验中。 低比活性的牛凝血酶以新西兰源牛血浆为原料制备凝血酶原,使用从新西兰源的牛肺组织中提取的凝血活酶激活凝血酶原,通过分离纯化获得,并在真空下冷冻干燥以粉末的形式供应。低比活力凝血酶的比活力为90-300 NIH U/mg蛋白,总蛋白含量大于50%。 凝血酶已用于评估感染性休克患者持续性低凝状态的研究。
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
SKU | 02199907-CF |
Alternate Names | Factor IIa; Thrombin from bovine plasma |
Application Notes | 凝血酶广泛应用于诊断行业中,用于凝血实验,凝血因子测试以及全血或血浆的去纤维化以用于血清的对照实验。凝血酶还可用于重组融合蛋白的特异性位点切割等生化实验中。 低比活性的牛凝血酶以新西兰源牛血浆为原料制备凝血酶原,使用从新西兰源的牛肺组织中提取的凝血活酶激活凝血酶原,通过分离纯化获得,并在真空下冷冻干燥以粉末的形式供应。低比活力凝血酶的比活力为90-300 NIH U/mg蛋白,总蛋白含量大于50%。 凝血酶已用于评估感染性休克患者持续性低凝状态的研究。 |
Base Catalog Number | 199907 |
Biochemical Physiological Actions | Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B. |
CAS # | 9002-04-4 |
EC Number | 232-648-7 |
Extinction Coefficient | E<sub>280</sub><sup>1%</sup> = 18.3 (human); E<sub>280</sub><sup>1%</sup> = 19.5 (bovine) |
Format | Lyophilized powder |
Grade | Analytical Grade |
Hazard Statements | H315-H319-H334-H335 |
Host | Bovine |
pH | 7.0 - 8.0 |
Protein or Enzyme Type | Plasma & Blood Proteins |
RTECS Number | XO8950000 |
Safety Symbol | GHS07, GHS08 |
Specific Activity | 90 - 300 u/mg protein |
Unit Definition | Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K. The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of albumin solution based on a modification of the method of Biggs. Only clotting times in the range of 15–25 seconds are used for determining thrombin activity. Optimal clotting temperature is 37 °C. |
Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |