Poly-L-Lysine Hydrobromide
Application Notes
Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates. Polymers of both D- and L-lysine are used to coat slides. However, certain cells can digest poly-L-lysine; in this situation, poly-D-lysine should be used as the attachment factor so that the cells are not disrupted by excessive uptake of L-lysine. The lower molecular weight poly-lysine (30,000-70,000) is easier to use because it is less viscous in solution; however, the higher molecular weights of poly-lysine have more attachement sites per molecule available to the cells. A compromise between the easier to use lower molecular weight products and the extremely viscous higher molecular weights would be the products in the range of 70,000-150,000.Other applications of Poly-L-lysine include Conjugation to methotrexate for increased drug transport,Microencapsulation of islets ,Use in simple reproducible procedure for chromosomal preparations from a variety of tissues,Immobilation of living cells in biocompatible semipermeable microcapsules,In the preparation of polycationic beads,Conjugation to albumin and horseradish peroxidase to enhance cellular uptake.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Attachment factor for tissue culture.
| SKU | 02150176-CF |
| Application Notes | Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates. Polymers of both D- and L-lysine are used to coat slides. However, certain cells can digest poly-L-lysine; in this situation, poly-D-lysine should be used as the attachment factor so that the cells are not disrupted by excessive uptake of L-lysine. The lower molecular weight poly-lysine (30,000-70,000) is easier to use because it is less viscous in solution; however, the higher molecular weights of poly-lysine have more attachement sites per molecule available to the cells. A compromise between the easier to use lower molecular weight products and the extremely viscous higher molecular weights would be the products in the range of 70,000-150,000.Other applications of Poly-L-lysine include Conjugation to methotrexate for increased drug transport,Microencapsulation of islets ,Use in simple reproducible procedure for chromosomal preparations from a variety of tissues,Immobilation of living cells in biocompatible semipermeable microcapsules,In the preparation of polycationic beads,Conjugation to albumin and horseradish peroxidase to enhance cellular uptake. |
| Base Catalog Number | 150176 |
| Biochemical Physiological Actions | Poly-L-lysine is a nonspecific attachment factor for cells useful in promoting cell adhesion to solid substrates by enhancing electrostatic interaction between negatively charged ions of the cell membrane and the culture surface. When it is absorbed to the cell culture surface, poly-L-lysine functions to increase the number of positively charged sites available for cell binding. With cells that can digest poly-L-lysine, poly-D-lysine should be used as the attachment factor. |
| CAS # | 25988-63-0 |
| Format | Powder |
| Molecular Weight | 402.54 g/mol |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [150176] lot ID: 3617K](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [150176] lot ID: 3617K")