Tryptic Soy Agar is a medium very rich in nutrients for "general use" in microbiological laboratories. Containing two peptones obtained by enzymatic hydrolysis of casein and soy protein, this medium supports the growth of a great variety of microorganisms, including fastidious aerobes and anaerobes. If desired, antibiotics can easily be incorporated as well as other supplements or inhibitory agents. A short list of microorganisms that grow on this medium are the following: Streptococcus, Neisseria, Brucella, Corynebacterium, Listeria, Pasteurella, Vibrio, Haemophilus vaginals, Candida, etc.
Application Notes
Tryptic Soy Agar is used for the isolation and cultivation of fastidious and non-fastidious micro-organisms. It supports the abundant growth of fastidious organisms such as Pneumococci, Streptococci, Neisserias, etc. Since it lacks carbohydrates, it is very useful in the study of hemolytic reactions and also in the preparation of chocolate agar. Also, it may be used for sterility testing. It can be used with or without blood supplement. It is used as precultivation medium and enumeration (E. coli) acc. to membrane-filter technique. As it does not contain the X and V factors, it is suitable for identification of Haemophilus sp. by adding X (Hemin) and V (DPN) factors strips.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Bacteria Cell Culture | Isolation and cultivation of fastidious organisms.
| SKU | 091010617 |
| Alternate Names | CASO Agar; Soybean Casein digest Agar; TSA; Tryptone Soya Agar; Trypticasein Soy Agar; Soyabean casein digest agar |
| Application Notes | Tryptic Soy Agar is used for the isolation and cultivation of fastidious and non-fastidious micro-organisms. It supports the abundant growth of fastidious organisms such as Pneumococci, Streptococci, Neisserias, etc. Since it lacks carbohydrates, it is very useful in the study of hemolytic reactions and also in the preparation of chocolate agar. Also, it may be used for sterility testing. It can be used with or without blood supplement. It is used as precultivation medium and enumeration (E. coli) acc. to membrane-filter technique. As it does not contain the X and V factors, it is suitable for identification of Haemophilus sp. by adding X (Hemin) and V (DPN) factors strips. |
| Base Catalog Number | 1010617 |
| Format | Powder |
| Formulation Details | Powder | Following is composition of Tryptic Soy Agar: Casein Peptone - 15 g/L; Sodium Chloride -5 g/L; Soy Peptone - 5 g/L; Agar - 15 g/L |
| Media Type | Tryptic Soy |
| Pack Size | 500 g |
| pH | 7.1 - 7.5 |
| Preparation Method | Suspend 40 g of the medium in one liter of deionized or distilled water. Heat with frequent agitation and boil for one minute. Sterilize in an autoclave between 118 and 121 °C at a pressure of no more than 15 lbs. for 15 minutes. In the case of large volume preparation, increase the time of sterilization but not the temperature or pressure. Cool and pour into Petri dishes. For hemolytic studies, add 5 to 10% sheep or rabbit blood. |
| Sterility | Sterilize in an autoclave between 118 and 121 °C at a pressure of no more than 15 lbs. for 15 minutes. |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [1010617] lot ID: 1162J](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [1010617] lot ID: 1162J")
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