L-Broth is a nutritionally rich medium originally developed for growth and maintenance of recombinant E. coli strains.

Application Notes

L-Broth Medium is a nutritionally rich medium originally developed for growth and maintenance of E. coli strains. The ultimate in ease and convenience, L-Broth Medium in a capsule form eliminates the waste, inaccuracies, and mess associated with weighing out bulk powder.The growth characteristics are the same as L-Broth powder. L-Broth Medium supports a luxurious growth of E. coli cells as tryptone and yeast extract supply essential growth factors that E. coli cells would otherwise have to synthesize. The medium also contains essential electrolytes for transport and osmotic balance, due to the NaCl component.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

SKU	113001021-CF
Alternate Names	Luria Broth Base
Application Notes	L-Broth Medium is a nutritionally rich medium originally developed for growth and maintenance of E. coli strains. The ultimate in ease and convenience, L-Broth Medium in a capsule form eliminates the waste, inaccuracies, and mess associated with weighing out bulk powder.The growth characteristics are the same as L-Broth powder. L-Broth Medium supports a luxurious growth of E. coli cells as tryptone and yeast extract supply essential growth factors that E. coli cells would otherwise have to synthesize. The medium also contains essential electrolytes for transport and osmotic balance, due to the NaCl component.
Base Catalog Number	3001021
Format	Capsules
Formulation Details	Typically contain 10 g Tryptone; 5 g Yeast Extract; 0.5 g NaCl. Small quantities of biological buffers have been added for proper pH adjustment.
Grade	Molecular Biology Certified™
Media Type	L-Broth
Personal Protective Equipment	Personal Protective Equipment Respiratory protection: Breathing apparatus only if aerosol or dust is formed. Hand protection: Pvc or other plastic material gloves Skin and body protection: Usual safety precautions while handling the product will provide adequate protection against this potential effect. Eye protection: Safety glasses with side-shields Hygiene measures: Handle in accordance with good industrial hygiene and safety practice.
pH	Acceptable pH range is 6.9 ± 0.5 at 25 °C. Please note that the pH of L-Broth Medium liquid increases as the temperature of the solution decreases.
Preparation Method	Media Preparation: Reagents are tested and chosen for their ability to promote optimum growth of E. coli when combined in L-Broth Medium formulations. Chosen reagents are combined in the proper proportions according to the L-Broth formulation and thoroughly blended for a uniform distribution. A sample of the blended formulation is used to prepare liquid medium. The combinations are mixed to dissolve components and then autoclaved at 121 °C for 15 minutes.
Solubility	Soluble in water (16 g/L).
Typical Working Concentration	16 g/L
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.