TBE buffer

Name: TBE buffer
SKU: 04816721-CF

CN¥0.00

Key features and details

Tris-Borate-EDTA electrophoresis buffer, ready-to-use

SKU: 04816721-CF

Synonyms

TBE buffer

CAS Number:

Not applicable

MDL Number:

MFCD00236358

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Product Description

Tris-Borate-Edta Buffer

Application Notes

TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. It is used to prepare a 1 X buffer for polyacrylamide and agarose gel electrophoresis. When diluted to a 1X concentration, it yields a TBE buffer solution that contains 100 mM Tris, 90 mM boric acid and 1mM EDTA. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Buffer for DNA and RNA polyacrylamide gel electrophoresis

Specifications

SKU	04816721-CF
Alternate Names	TBE buffer
Application Notes	TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids. It is used to prepare a 1 X buffer for polyacrylamide and agarose gel electrophoresis. When diluted to a 1X concentration, it yields a TBE buffer solution that contains 100 mM Tris, 90 mM boric acid and 1mM EDTA. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.
Base Catalog Number	816721
CAS #	Not applicable
Format	Solution
Hazard Statements	H360FD
Personal Protective Equipment	Eyeshields, full-face respirator, Gloves, multi-purpose combination respirator cartridge, respirator filter
Safety Symbol	GHS08
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.