Lysozyme type VI, molecular biology reagent, ≥23,500 Shugar units/mg

Name: Lysozyme type VI, molecular biology reagent, ≥23,500 Shugar units/mg
SKU: 02195303-CF

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Key features and details

Lysozyme Type Vi

SKU: 02195303-CF

Synonyms

Muramidase; Mucopeptide N-acetylmuramoylhydrolase

CAS Number:

9001-63-2

Molecular Weight:

2235.597 g/mol

EC Number:

232-620-4

MDL Number:

MFCD00131557

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Product Description

Lysozyme Type Vi

Application Notes

Lysozyme has been used for lysing E. coli and Streptomycetes for extraction purposes such as extracting group specific antigen. It would appear that lysozyme may act as a germinative agent of bacterial spores.In humans, lysozyme may be the mediator in the anti-tumor function of macrophages which, it has been shown, secrete the enzyme. There is evidence that cartilage lysozyme has a role in cartilage calcification.

Usage Statement

Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.

Key Applications

Cell Lysis

Specifications

SKU	02195303-CF
Alternate Names	Muramidase; Mucopeptide N-acetylmuramoylhydrolase
Application Notes	Lysozyme has been used for lysing E. coli and Streptomycetes for extraction purposes such as extracting group specific antigen. It would appear that lysozyme may act as a germinative agent of bacterial spores.In humans, lysozyme may be the mediator in the anti-tumor function of macrophages which, it has been shown, secrete the enzyme. There is evidence that cartilage lysozyme has a role in cartilage calcification.
Base Catalog Number	195303
Biochemical Physiological Actions	Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane. The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).
CAS #	9001-63-2
EC Number	232-620-4
Extinction Coefficient	26.4 (280 nm) (Lit.)
Format	lyophilized, Salt-free, 3X Crystallized, Albumin-Free, Type VI
Grade	Molecular Biology Grade
Isoelectric Point	pH 11.0 (lit)
Molecular Weight	2235.597 g/mol
Personal Protective Equipment	Eyeshields, Gloves, respirator filter
RTECS Number	OL5987000
Source	Chicken eggwhite
Specific Activity	≥23,500 Shugar units/mg
Unit Definition	One unit will produce a decrease in A450 of 0.001 per minute at pH 6.24 and 25 °C using Micrococcus lysodeikticus as substrate.
Usage Statement	Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.