The MagBeads FastDNA® Kit for Soil allows quick and efficient isolation of high-quality genomic DNA from soil in less than 60 mins. Samples are placed into Lysing Matrix E tube and used with FastPrep® Instruments from MP Biomedicals to effectively lyse host cells as well as bacteria, fungi, viruses, protists, and other cells present in soil samples within 40 seconds. It consists of specially formulated reagents to eliminate humic acid, polysaccharides, phenolic compounds, and enzyme inhibitors from soil and thus allowing extraction of highly pure genomic DNA ready for PCR, restriction digestion, electrophoresis, and other desired applications.
Performance
MagBeads FastDNA® Kit for Soil has been extensively tested for its performance. The following figures show the gDNA yields obtained from various soil samples using the kit. Results demonstrate high yields and purity of extracted gDNA, ready-to-use for PCR amplification.
|
|
|
 |
|
Figure 1: gDNA extracted from various soil samples using MagBeads FastDNA® Kit for Soil, analyzed using 1% agarose gel electrophoresed at 70 V for 30 mins. M: 1kb plus DNA ladder; Lane 1-4: Automation extraction; Lane 5-8: Manual extraction; Lane 1: Organic soil 3 µL; Lane 2: Flowerbed soil 8 µL; Lane 3: Saline soil 8 µL; Lane 4: Desert soil 8 µL. |
|
Figure 2: PCR amplification of 16S rRNA gene from various soil samples using MagBeads FastDNA® Kit for Soil, analyzed using 1% agarose gel electrophoresed at 70 V for 30 mins. M: 1kb plus DNA ladder; Lane 1: Organic soil; Lane 2: Flowerbed soil; Lane 3: Saline soil; Lane 3: Desert soil; Lane 5: Negative control. |
Application Notes
MagBeads FastDNA® Kit for Soil allows quick and efficient isolation of high-quality genomic DNA from soil in less than 60 mins. It consists of specially formulated reagents to eliminate humic acid, polysaccharides, phenolic compounds, and enzyme inhibitors from soil and thus allowing extraction of highly pure genomic DNA ready for PCR, restriction digestion, electrophoresis, and other desired applications.
Usage Statement
Research Use Only (RUO).
Key Applications
Genomic, Molecular Biology, PCR, qPCR, Soil
| SKU | 117034100 |
| Application Notes | MagBeads FastDNA® Kit for Soil allows quick and efficient isolation of high-quality genomic DNA from soil in less than 60 mins. It consists of specially formulated reagents to eliminate humic acid, polysaccharides, phenolic compounds, and enzyme inhibitors from soil and thus allowing extraction of highly pure genomic DNA ready for PCR, restriction digestion, electrophoresis, and other desired applications. |
| Base Catalog Number | 7034100 |
| Components | Lysing Matrix E, Lysis Buffer S1, Lysis Buffer S2, RNase A Solution, Inhibitor Removal MS, Binding Buffer MS, Magnetic Beads, Wash Buffer S1, Wash Buffer S2, DES Buffer, 96 Spin Tips |
| Pack Size | 96 Preps |
| Personal Protective Equipment | Safety Goggles/Faceshield; Gloves |
| Storage | All MagBeads FastDNA® Kit for Soil components are guaranteed for at least 24 months from the date of manufacture when stored as follows. Store Magnetic Beads at 2 to 8 ˚C. Store all other components at room temperature (15 to 25˚C). |
| Usage Statement | Research Use Only (RUO). |
![MP COA [117034100] lot ID: FC12007](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [117034100] lot ID: FC12007")