Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. It has proven effective in the culture of tissue derived from moncotyledons and dicotyledons. This medium, originally formulated to support tobacco callus, has been used to support callus initiation and growth, the growth of cells in suspension culture and the regeneration of shoots and plantlets from explants. More recently, it has been used for micropropagation of ornamental, vegetable and fruit plants in research and commercial enterprises.
Application Notes
Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. It has proven effective in the culture of tissue derived from moncotyledons and dicotyledons. This medium, originally formulated to support tobacco callus, has been used to support callus initiation and growth, the growth of cells in suspension culture and the regeneration of shoots and plantlets from explants. More recently, it has been used for micropropagation of ornamental, vegetable and fruit plants in research and commercial enterprises.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Plant Cell Growth | Plant tissue culture growth
SKU | 0926230-CF |
Application Notes | Murashige and Skoog medium is a widely used plant tissue culture growth medium. M&S Basal Medium contains macronutrients that include high levels of nitrate and organic additives such as agar, sugars, vitamins and growth regulators. It has proven effective in the culture of tissue derived from moncotyledons and dicotyledons. This medium, originally formulated to support tobacco callus, has been used to support callus initiation and growth, the growth of cells in suspension culture and the regeneration of shoots and plantlets from explants. More recently, it has been used for micropropagation of ornamental, vegetable and fruit plants in research and commercial enterprises. |
Base Catalog Number | 2623020,2623022 |
Format | Solution |
Formulation Details | Powder | Components: Ammonium Nitrate: 1650.0 mg/L; Boric Acid: 6.2 mg/L; Calcium Chloride; Anhyd.: 332.2 mg/L; Cobalt Chloride 6H<sub>2</sub>O: 0.025 mg/L; Cupric Sulfate 5H<sub>2</sub>O: 0.025 mg/L; Na<sub>2</sub>- EDTA: 37.26 mg/L; Ferrous Sulfate 7H<sub>2</sub>O: 27.8 mg/L; Magnesium Sulfate: 180.7 mg/L; Manganese Sulfate: 16.9 mg/L; Molybdic Acid; Sodium Salt; 2H<sub>2</sub>O: 0.25 mg/L; Potassium Iodide: 0.83 mg/L; Potassium Nitrate: 1900.0 mg/L; Potassium Phosphate; Monobasic: 170.0 mg/L; Zinc Sulfate; 7H<sub>2</sub>O: 8.60 mg/L; Grams of powder to prepare 1 liter: 4.3 mg/L. |
Media Type | Murashige and Skoog |
pH | 3.5 - 4.5 (4.33 g/L) |
Solubility | Soluble in water (1×, Clear, Colorless to Clear with Slight Yellow Tint). Soluble in water (4.331 g/L). |
Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |