N,N,N′,N′-Tetramethylethylenediamine
Application Notes
N,N,N',N'-Tetramethylethylenediamine is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. The free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for sieving macromolecules such as nucleic acids and proteins. TEMED has been used in the coating for soft polymers, such as polyacrylamide, in capillaries for investigation by atom force microscopy. Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED. It is also used used in the HPLC of small organic molecules.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Polyacrylamide gel formation catalyst
| SKU | 02194019-CF |
| Alternate Names | TEMED; TMEDA; 1,2-Bis(dimethylamino)ethane |
| Application Notes | N,N,N',N'-Tetramethylethylenediamine is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. The free radicals will cause acrylamide and bis-acrylamide to polymerize to form a gel matrix which can be used for sieving macromolecules such as nucleic acids and proteins. TEMED has been used in the coating for soft polymers, such as polyacrylamide, in capillaries for investigation by atom force microscopy. Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED. It is also used used in the HPLC of small organic molecules. |
| Base Catalog Number | 194019 |
| Beilstein Registry Number | 1732991 |
| Boiling Point | 250 to 252° F at 760 mm Hg (NTP, 1992) |
| CAS # | 110-18-9 |
| Density | 0.7765 at 68° F (NTP, 1992) |
| EC Number | 203-744-6 |
| Flash Point | 50° F (NTP, 1992) |
| Format | Liquid |
| Grade | Molecular Biology Grade |
| Hazard Statements | H225-H302-H314-H332 |
| Melting Point | -67.2° F (NTP, 1992) |
| Molecular Formula | C6H16N2 |
| Molecular Weight | 116.208 g/mol |
| Personal Protective Equipment | Faceshields, full-face respirator , Gloves, Goggles, multi-purpose combination respirator cartridge,respirator filter |
| Purity | ~99% |
| Refractive Index | n20/D 1.4179 (20 °C)(Lit.) |
| RTECS Number | KV7175000 |
| Safety Symbol | GHS02, GHS05, GHS07 |
| Solubility | Soluble (NTP, 1992) |
| Storage | 2-8°C |
| Typical Working Concentration | Suggested use is 6-10 uL for 10 mL acrylamide gel solution (the less amount of TEMED used, the slower the polymerization) |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
![MP COA [194019] lot ID: 1385B](https://www.mpbiochina.com/static/version1714032662/frontend/Mpbio/default/en_US/images/document.png "MP COA [194019] lot ID: 1385B")
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