Nuclease P1
Application Notes
Nuclease P1 is useful at removing single stranded strands hanging off the end of double stranded DNA and at completely cleaving melted DNA for simple DNA composition analysis.Nuclease P1 from Penicillium citrinum has been used in a study to assess crystal structures using ammonium sulphate or polyethylene glycol 4000 as a precipitating agent. It has also been used in a study to investigate a method for the direct sequence analysis 20-25 nucleotides from the terinini of 5' or 3' end group labeled RNA. Nuclease P1 is used to improve the sensitivity of a 32P-labeling method for the detection of DNA adducts.
Usage Statement
Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department.
Key Applications
Endonuclease
| SKU | 0218055501 |
| Alternate Names | Nucleate 5'-nucleotidohydrolase (3'-phosphohydrolase);Penicillium Nuclease P1; EC 3. 1. 30.1; Deoxyribonuclease P1; Endonuclease P1 |
| Application Notes | Nuclease P1 is useful at removing single stranded strands hanging off the end of double stranded DNA and at completely cleaving melted DNA for simple DNA composition analysis.Nuclease P1 from Penicillium citrinum has been used in a study to assess crystal structures using ammonium sulphate or polyethylene glycol 4000 as a precipitating agent. It has also been used in a study to investigate a method for the direct sequence analysis 20-25 nucleotides from the terinini of 5' or 3' end group labeled RNA. Nuclease P1 is used to improve the sensitivity of a 32P-labeling method for the detection of DNA adducts. |
| Base Catalog Number | 18055501 |
| Biochemical Physiological Actions | Catalyzes the nonspecific endonucleolytic cleavage of single stranded DNA and RNA to yield nucleoside 5′-phosphates and 5′-phosphooligonucleotides. It does not appreciably degrade double-stranded nucleic acids, especially in the presence of more than 400 mM sodium chloride at pH 6.0. |
| CAS # | 54576-84-0 |
| Format | Solid |
| Pack Size | 500 U |
| Personal Protective Equipment | Eyeshields, Gloves, respirator filter |
| Source | Penicillin citrinum |
| Specific Activity | ~500 units/vial |
| Unit Definition | The amount of enzyme that produces 1 µmole of acid-soluble neucleotides from RNA based on E=10,600 for RNA hydrolysates per minute at 37 °C, pH 5.3. One unit of the enzyme liberates ~ 4 mmoles of orthophosphate from 3'-AMP per minute at 37 °C, pH 7.2 |
| Usage Statement | Unless specified otherwise, MP Biomedical's products are for research or further manufacturing use only, not for direct human use. For more information, please contact our customer service department. |
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